Supplementing beef cattle with 3-nitrooxypropanol (3-NOP) decreases enteric methane production, but it is unknown if fertilizing soil with 3-NOP manure influences soil health. We measured soil health indicators 2 yr after manure application to a bromegrass (Bromus L.) and alfalfa (Medicago sativa L.) mixed crop. Treatments were: composted conventional manure (without supplements); stockpiled conventional manure; composted manure from cattle supplemented with 3-NOP; stockpiled 3-NOP manure; composted manure from cattle supplemented with 3-NOP and monensin (3-NOP+Mon), a supplement that improves digestion; stockpiled 3-NOP+Mon manure; inorganic fertilizer (150 kg N ha^–1 and 50 kg P ha^–1); and an unamended control. Select chemical (K⁺, Mg²⁺, Mn⁺, Zn⁺, pH, and Olsen-P), biological (soil organic matter, active C, respiration, and extractable protein), physical (wet aggregate stability, bulk density, total porosity, and macro-, meso-, and micro-porosity), and hydraulic (saturation, field capacity, wilting point, water holding capacity, and hydraulic conductivity) variables were measured. The inclusion of monensin decreased soil Zn⁺ concentrations by 70% in stockpiled 3-NOP+Mon compared with stockpiled conventional manure. Active C and protein in composted conventional manure were 37 and 92% higher compared with stockpiled manure, respectively, but did not vary between 3-NOP treatments. 3-Nitrooxypropanol did not significantly alter other soil health indicators. Our results suggest that composted and stockpiled 3-NOP manure can be used as a nutrient source for forage crops without requiring changes to current manure management because it has minimal influence on soil health.

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3-硝基氧丙醇肥料对土壤健康和水力特性的影响

用 3-硝基氧丙醇 (3-NOP) 补充肉牛会降低肠道甲烷的产生，但尚不清楚用 3-NOP 粪便施肥土壤是否会影响土壤健康。我们在对雀麦( Bromus L.) 和苜蓿 ( Medicago sativa L.) 混合作物施肥 2 年后测量了土壤健康指标。处理方法是： 堆肥常规肥料（无补充剂）；储存的常规肥料；来自牛的堆肥肥料，并添加了 3-NOP；储存的 3-NOP 粪便；来自牛的堆肥肥料，添加了 3-NOP 和莫能菌素 (3-NOP+Mon)，这是一种改善消化的补充剂；储存 3-NOP+Mon 粪肥；无机肥料（150 kg N ha ^–1和 50 kg P ha ^–1）；和未经修改的控制。选择化学品（K⁺、Mg ²⁺、Mn ⁺、Zn ⁺、pH 和 Olsen-P）、生物（土壤有机质、活性碳、呼吸作用和可提取蛋白质）、物理（湿聚集体稳定性、堆积密度、总孔隙率和宏观-、中孔和微孔）和水力（饱和度、田间持水量、枯萎点、持水量和水力传导率）变量进行了测量。莫能菌素的加入降低了土壤Zn ⁺与储存的常规粪便相比，储存的 3-NOP+Mon 中的浓度降低了 70%。与堆肥相比，堆肥常规粪便中的活性 C 和蛋白质分别高出 37% 和 92%，但在 3-NOP 处理之间没有变化。3-硝基氧丙醇没有显着改变其他土壤健康指标。我们的结果表明，堆肥和储存的 3-NOP 粪便可用作饲料作物的营养来源，而无需改变当前的粪便管理，因为它对土壤健康的影响最小。