Electrospun membranes are attracting interest as a drug delivery system because of their material composition flexibility and versatile drug loading. In this study, the electrospun membrane was loaded with doxorubicin (DOX) via electrostatic adsorption for long-term drug delivery. DOX loading process was optimized by varying temperature, time, drug concentration, pH and ionic strength of solutions. The loading process did not impair the structural properties of the membrane. Next, we investigated the drug release kinetics using spectroscopic techniques. The composite membranes released 22% of the adsorbed DOX over the first 48 h, followed by a slower and sustained release over 4 weeks. The DOX release was sensitive to acidic solutions that the release rate at pH 6.0 was 1.27 times as that at pH 7.4. The DOX-loaded membranes were found to be cytotoxic to U-87 MG cells in vitro that decreased the cell viability from 82.92% to 25.49% from 24 to 72 h of co-incubation. These membranes showed strong efficacy in suppressing tumour growth in vivo in glioblastoma-bearing mice that decreased the tumour volume by 77.33% compared with blank membrane-treated group on Day 20. In conclusion, we have developed an effective approach to load DOX within a clinically approved poly (L-lactic acid)/gelatine membrane for local and long-term delivery of DOX for the treatment of glioblastoma.

中文翻译：

---

负载阿霉素的电纺聚（L-乳酸）/明胶膜可有效抑制体外和体内胶质母细胞瘤细胞的生长


电纺膜作为药物输送系统因其材料成分的灵活性和多功能的载药量而引起人们的兴趣。在这项研究中，电纺膜通过静电吸附负载阿霉素（DOX），用于长期药物输送。通过改变溶液的温度、时间、药物浓度、pH 值和离子强度来优化 DOX 装载过程。加载过程不会损害膜的结构性能。接下来，我们使用光谱技术研究了药物释放动力学。复合膜在前 48 小时内释放了 22% 的吸附 DOX，随后在 4 周内缓慢且持续地释放。 DOX释放对酸性溶液敏感，pH 6.0时的释放速率是pH 7.4时的1.27倍。研究发现，负载 DOX 的膜在体外对 U-87 MG 细胞具有细胞毒性，在共孵育 24 至 72 小时期间，细胞活力从 82.92% 降低至 25.49%。这些膜在抑制胶质母细胞瘤小鼠体内肿瘤生长方面表现出强大的功效，与空白膜治疗组相比，第 20 天的肿瘤体积减少了 77.33%。 总之，我们开发了一种在临床上负载 DOX 的有效方法。批准聚（L-乳酸）/明胶膜用于局部和长期输送 DOX 治疗胶质母细胞瘤。