Electrospun membranes are attracting interest as a drug delivery system because of their material composition flexibility and versatile drug loading. In this study, the electrospun membrane was loaded with doxorubicin (DOX) via electrostatic adsorption for long-term drug delivery. DOX loading process was optimized by varying temperature, time, drug concentration, pH and ionic strength of solutions. The loading process did not impair the structural properties of the membrane. Next, we investigated the drug release kinetics using spectroscopic techniques. The composite membranes released 22% of the adsorbed DOX over the first 48 h, followed by a slower and sustained release over 4 weeks. The DOX release was sensitive to acidic solutions that the release rate at pH 6.0 was 1.27 times as that at pH 7.4. The DOX-loaded membranes were found to be cytotoxic to U-87 MG cells in vitro that decreased the cell viability from 82.92% to 25.49% from 24 to 72 h of co-incubation. These membranes showed strong efficacy in suppressing tumour growth in vivo in glioblastoma-bearing mice that decreased the tumour volume by 77.33% compared with blank membrane-treated group on Day 20. In conclusion, we have developed an effective approach to load DOX within a clinically approved poly (L-lactic acid)/gelatine membrane for local and long-term delivery of DOX for the treatment of glioblastoma.

中文翻译：

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负载多柔比星的电纺聚（L-乳酸）/明胶膜在体外和体内有效抑制胶质母细胞瘤细胞的生长

静电纺丝膜因其材料组成的灵活性和多功能的载药量而作为药物输送系统引起了人们的兴趣。在这项研究中，静电纺丝膜通过静电吸附装载了阿霉素 (DOX)，用于长期药物输送。通过改变溶液的温度、时间、药物浓度、pH 和离子强度来优化 DOX 加载过程。加载过程不会损害膜的结构特性。接下来，我们使用光谱技术研究了药物释放动力学。复合膜在前 48 小时内释放了 22% 的吸附 DOX，随后在 4 周内缓慢而持续地释放。DOX 的释放对酸性溶液敏感，pH 6.0 的释放速率是 pH 7.4 的 1.27 倍。发现载有 DOX 的膜在体外对 U-87 MG 细胞具有细胞毒性，从 24 到 72 小时的共孵育将细胞活力从 82.92% 降低到 25.49%。这些膜在抑制胶质母细胞瘤小鼠体内肿瘤生长方面表现出很强的功效，在第 20 天，与空白膜处理组相比，肿瘤体积减少了 77.33%。总之，我们已经开发出一种有效的方法在临床上加载 DOX批准聚（L-乳酸）/明胶膜用于局部和长期递送 DOX 治疗胶质母细胞瘤。