Background. Telocytes (TCs) are a distinct type of interstitial cells that play a vital role in the pathogenesis of ulcerative colitis and colonic tissue hemostasis. The aim of this study was to examine the effect of nanocurcumin (NC) on the morphometric and immunohistochemical characterization of TCs in the ulcerative colitis (UC) rat model. Methods. Forty rats were randomly divided into control, NC, UC, and UC+NC groups. At the end of the experiment, the colon was dissected and prepared for histopathological and immunohistochemical assessment. Tissue homogenates were prepared for real-time PCR assessment of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-beta (TGF-β) gene expression. Our results revealed extensive mucosal damage with inflammatory cell infiltration, significant reduction of CD34, and vimentin immunostained TCs in the colon of the UC group with significant elevation of expression of IL-6, TNF-α, and TGF-β. The UC+NC-treated group revealed significant elevation of TC count compared to the UC group besides, a significant reduction of the three gene expression. Conclusion. NC successfully targeted the colonic tissue, improved the mucosal lesion, preserve TCs distribution, and count through its anti-inflammatory and fibrinolytic properties.

中文翻译：

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溃疡性结肠炎大鼠模型结肠内纳米姜黄素诱导的远程细胞保存的分子基础的鉴定

背景。端粒细胞 (TCs) 是一种独特类型的间质细胞，在溃疡性结肠炎的发病机制和结肠组织止血中起重要作用。本研究的目的是检查纳米姜黄素 (NC) 对溃疡性结肠炎 (UC) 大鼠模型中 TC 的形态学和免疫组织化学表征的影响。方法。40只大鼠随机分为对照组、NC组、UC组和UC+NC组。在实验结束时，解剖结肠并准备进行组织病理学和免疫组织化学评估。制备组织匀浆用于白细胞介素 6 (IL-6)、肿瘤坏死因子-α (TNF- α ) 和转化生长因子-β (TGF- β )的实时 PCR 评估） 基因表达。我们的结果显示，UC 组结肠中广泛的黏膜损伤伴有炎症细胞浸润，CD34 和波形蛋白免疫染色的 TCs 显着升高，IL-6、TNF- α和 TGF- β的表达显着升高。与 UC 组相比，UC+NC 治疗组显示出 TC 计数显着升高，此外三种基因表达显着降低。结论。NC 成功地靶向结肠组织，改善粘膜病变，保持 TC 分布，并通过其抗炎和纤溶特性进行计数。