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Photobiomodulation therapy improved functional recovery and overexpression of interleukins-10 after contusion spinal cord injury in rats
Journal of Chemical Neuroanatomy ( IF 2.7 ) Pub Date : 2021-07-31 , DOI: 10.1016/j.jchemneu.2021.102010
Mahnaz Poor Hassan 1 , Mohammad-Amin Abdollahifar 2 , Abbas Aliaghaei 3 , Faraj Tabeie 4 , Saeed Vafaei-Nezhad 5 , Mohsen Norouzian 2 , Hojjat Allah Abbaszadeh 6
Affiliation  

Following severe Spinal Cord Injury (SCI), regeneration is inadequate, and functional recovery is incomplete. The occurrence of oxidative stress and the spread of inflammation play a crucial role in the failure to regenerate the injury site. In this way, we explored the neuroprotective effects of PhotoBioModulation (PBM), as the main factor in controlling these two destructive factors, on SCI. fifty-four female adult Wistar rats divided into three groups: sham group (just eliminate vertebra lamina, n = 18), SCI group (n = 18), and SCI-PBM group which exposed to PBM (150 MW, 50 min/day, 14 days, n = 18). After SCI induction at the endpoint of the study (the end of 8 week), we took tissue samples from the spinal cord for evaluating the biochemical profiles that include Catalase (CAT), Malondialdehyde (MDA), Superoxide Dismutase (SOD), Glutathione Peroxidase (GSH-PX) levels, immunohistochemistry for Caspase-3, gene expressions of Interleukin-1β (IL-1β), Tumor Necrosis Factor-alpha (TNF-α), and Interleukin (IL-10). Also, stereological assessments evaluated the spinal cord, central cavity volumes, and numerical density of the glial and neural cells in the traumatic area. The open-field test, rotarod test, Narrow Beam Test (NBT), Electromyography recording (EMG) test and the Basso–Beattie–Bresnehan (BBB) evaluated the neurological functions. Our results showed that the stereological parameters, biochemical profiles (except MDA), and neurological functions were markedly greater in the SCI-PBM group in comparison with SCI group. The transcript for the IL-10 gene was seriously upregulated in the SCI-PBM group compared to the SCI group. This is while gene expression of TNF-α and IL-1β, also density of apoptosis cells in Caspase-3 evaluation decreased significantly more in the SCI-PBM group compared to the SCI group. Overall, using PBM treatment immediately after SCI has neuroprotective effects by controlling oxidative stress and inflammation and preventing the spread of damage.



中文翻译:

光生物调节疗法改善大鼠脊髓挫伤后IL-10的功能恢复和过表达

严重脊髓损伤 (SCI) 后,再生不足,功能恢复不完整。氧化应激的发生和炎症的扩散在损伤部位再生失败中起着至关重要的作用。通过这种方式,我们探索了 PhotoBioModulation (PBM) 作为控制这两种破坏性因素的主要因素对 SCI 的神经保护作用。将 54 只雌性成年 Wistar 大鼠分为三组:假手术组(仅消除椎板,n = 18)、SCI 组(n = 18)和暴露于 PBM(150 MW,50 分钟/天)的 SCI-PBM 组, 14 天, n = 18)。在研究终点(第 8 周结束)进行 SCI 诱导后,我们从脊髓中采集组织样本以评估包括过氧化氢酶 (CAT)、丙二醛 (MDA)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-PX) 水平、Caspase-3 的免疫组织化学、白细胞介素 1β (IL-1β)、肿瘤坏死因子-α (TNF-α) 和白细胞介素 (IL-10) 的基因表达。此外,体视评估评估了创伤区域中神经胶质细胞和神经细胞的脊髓、中央腔体积和数值密度。开放场测试、旋转棒测试、窄束测试 (NBT)、肌电图记录 (EMG) 测试和 Basso-Beattie-Bresnehan (BBB) 评估神经功能。我们的结果表明,与 SCI 组相比,SCI-PBM 组的体视学参数、生化特征(MDA 除外)和神经功能显着提高。与 SCI 组相比,SCI-PBM 组中 IL-10 基因的转录物严重上调。这是 TNF-α 和 IL-1β 的基因表达,与 SCI 组相比,SCI-PBM 组中 Caspase-3 评估中凋亡细胞的密度也显着降低。总体而言,在 SCI 后立即使用 PBM 治疗通过控制氧化应激和炎症以及防止损伤扩散具有神经保护作用。

更新日期:2021-08-12
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