Considering the intense trend towards nutraceuticals and functional foods enriched with natural additives, and the great development in the cosmetic industry, it is obvious that it is necessary to study on the extraction of natural products. The current challenge is to develop the most effective alternative method by employing less energy, chemicals and unit operations. In the present study, automatic solvent extraction (AMSE) has been operated as a Green Extraction technology. Olive tree (Olea europaea) by-product (leaf) has been extracted by AMSE. Its yield has been evaluated according to its total phenolic (TPC), flavonoid (TFC) and oleuropein contents. Free radical scavenging activity of the product was also quantified by two different in vitro tests (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)). 3-factor and 3-level Central Composite Design (CCD) was employed to design the experimental study as well as modelling the experimental data and optimizing the AMSE process. The concentration of ethanol (solvent solution) was statistically the most significant (p < 0.0001) parameter affecting the AMSE of bioactive ingredients from olive leaves. The optimal conditions (~0.7 mm olive leaf particle with 80 % (v/v) ethanol solution for 30 min) have been determined to achieve the highest yields (216.39 mg gallic acid equivalent of TPC, 338.21 mg catechin equivalent of TFC and 67.25 mg of oleuropein per gram dried leaf), which are in convincing agreement with the actual values (<2 % error).

考虑到富含天然添加剂的营养保健品和功能性食品的强烈趋势，以及化妆品行业的巨大发展，显然有必要研究天然产品的提取。当前的挑战是通过使用更少的能源、化学品和单元操作来开发最有效的替代方法。在本研究中，自动溶剂萃取 (AMSE) 已作为绿色萃取技术进行操作。AMSE 提取了橄榄树 ( Olea europaea ) 的副产品（叶）。其产量已根据其总酚 (TPC)、类黄酮 (TFC) 和橄榄苦苷含量进行评估。产品的自由基清除活性也通过两种不同的体外定量测试（2,2-二苯基-1-苦基肼（DPPH）和2,2'-叠氮基-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS））。3 因子和 3 级中央复合设计 (CCD) 被用来设计实验研究以及建模实验数据和优化 AMSE 过程。乙醇（溶剂溶液）的浓度在统计上最显着（p < 0.0001) 影响橄榄叶生物活性成分 AMSE 的参数。已确定最佳条件（~0.7 mm 橄榄叶颗粒与 80% (v/v) 乙醇溶液 30 分钟）可实现最高产量（216.39 mg 没食子酸相当于 TPC，338.21 mg 儿茶素相当于 TFC 和 67.25 mg每克干叶的橄榄苦苷含量），这与实际值令人信服地一致（<2% 误差）。