Aiming to reveal the humification process of organic waste and its contribution to the heavy metal behaviour affected by earthworm activity, it was studied about the variation of humic acid (HA) and heavy metal behaviour during vermicomposting of the mixed pig manure and ¹³C-labelled rice straw. The results showed that earthworms could well adapt to the culturing environment and feed organic matter for its growth and reproduction, the vermicomposting process increased the content of humic substances (HS), HA, and fulvic acid (FA) in substrate residues, but led to less transformation of HA into FA. The elemental, ultraviolet absorption spectroscopy, Fourier transform infrared (FTIR) and fluorescence excitation–emission matrix (EEM) analysis indicated that vermicomposting led to more aromatic structures and much higher humification degree in HA, whereas less protein, FA-like substances and plastein in HA. Vermicomposting could enhance the total Cu content and decrease Cu/Zn bioavailability in the substrate residues, and vermicomposting especially can help stabilize Cu in the substrate residues by forming more complexed HA–Cu.

为揭示有机废弃物腐殖化过程及其对蚯蚓活动影响重金属行为的贡献，研究了混合猪粪和^{13蚯蚓堆肥过程中腐殖酸（HA）和重金属行为的变化。}C标记的稻草。结果表明，蚯蚓能够很好地适应养殖环境并为其生长繁殖提供有机物，蚯蚓堆肥过程增加了基质残渣中腐殖质（HS）、HA和黄腐酸（FA）的含量，但导致HA 向 FA 的转化较少。元素、紫外吸收光谱、傅里叶变换红外（FTIR）和荧光激发-发射矩阵（EEM）分析表明，蚯蚓堆肥导致HA中更多的芳香结构和更高的腐殖化程度，而更少的蛋白质、类FA物质和plastein哈。蚯蚓堆肥可以提高总铜含量并降低底物残留物中的铜/锌生物利用度，