American Journal of Alzheimer's Disease and other Dementias ( IF 3.4 ) Pub Date : 2021-07-31 , DOI: 10.1177/15333175211027681 Yuchen He 1 , Yi Qiang 2
This paper studied the influence of exercise on the cognitive ability of AD patients and elucidated potential mechanisms. The expression of SNHG14 was validated by qRT-PCR. The cognitive impairment of mice was examined by MWM Test. ELISA tests were applied to discover the influence of SNHG14 on inflammation. Overexpression of SNHG14 was found in AD patients and underexpression of SNHG14 was identified in these AD patients after exercise. In APP/PS1 double transgenic mice, SNHG14 reversed the protective impacts of exercise on escape latency and distance moved. The upregulation of SNHG14 also inhibited the effects of exercise on the percentage of time spent in the target quadrant and times of platform crossing. Besides, overexpression of SNHG14 reversed the repressed expression of IL-6, IL-1β, and TNF-α. In total, exercise could ameliorate cognitive disorder and inflammation activity by reducing the levels of SNHG14.
中文翻译:
自主运动通过调控lncRNA SNHG14改善阿尔茨海默病认知功能的机制
本文研究了运动对AD患者认知能力的影响,并阐明了可能的机制。通过qRT-PCR验证SNHG14的表达。通过MWM测试检查小鼠的认知障碍。ELISA 测试用于发现 SNHG14 对炎症的影响。在 AD 患者中发现 SNHG14 过表达,在这些 AD 患者中发现运动后 SNHG14 表达不足。在 APP/PS1 双转基因小鼠中,SNHG14 逆转了运动对逃避潜伏期和移动距离的保护作用。SNHG14 的上调也抑制了运动对目标象限所花费时间百分比和平台穿越次数的影响。此外,SNHG14 的过表达逆转了 IL-6、IL-1β 和 TNF-α 的抑制表达。总共,