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Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-07-30 , DOI: 10.1002/jemt.23891 Lorrane de Andrade Pereira 1, 2 , Margareth Maria de Carvalho Queiroz 2 , Suzana Côrte-Real Faria 3 , Viviane Zahner 2
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-07-30 , DOI: 10.1002/jemt.23891 Lorrane de Andrade Pereira 1, 2 , Margareth Maria de Carvalho Queiroz 2 , Suzana Côrte-Real Faria 3 , Viviane Zahner 2
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Brevibacillus laterosporus has entomopathogenic potential against several orders of insects and its wide bioactivity is associated with a variety of strain-specific molecules. In order to avoid the use of synthetic insecticides, along with the need to control insect pests, microbial control has been widely used. Muscoid dipterans are known for their medical-veterinary and sanitary importance, and synanthropy. The enormous biotechnological potential of B. laterosporus has been demonstrated, but there are still few studies with muscoid dipterans. The aim of the study was to verify the mortality of B. laterosporus NRS590 on synanthropic flies and to characterize its different cell stages ultrastructurally. The flies were collected from garbage bins and the colonies were adapted to the laboratory conditions. Bioassays with neo larvae were carried out from the bacterial growth in the phases: vegetative (6 hr), sporangium (20 hr), and free spores (44 hr). An aliquot of each phase was collected for Transmission (TEM) and Scanning Electron Microscopy (SEM). The effectiveness of NRS590 was observed in the sporulation phase, where the corrected mortality was 83.3, 85.1, and 99% for Chrysomya megacephala, Chrysomya putoria, and Musca domestica, respectively. The parasporal body was observed in detail on the entire spore surface. Although our knowledge of this bacterium is growing, it remains to be determined the real virulence factors responsible for the wide entomopathogenic activity observed on muscoid dipterans. Therefore, this study can provide subsidies for the improvement of efficient and safe microbial control techniques for the environment and living beings.
中文翻译：
侧孢短芽孢杆菌的超微结构和致病性对正嗜性粘液双翅目
侧孢短芽孢杆菌对几种昆虫具有致病潜力，其广泛的生物活性与多种菌株特异性分子有关。为了避免使用合成杀虫剂，以及防治害虫的需要，微生物防治已被广泛使用。双翅类粘虫以其医学兽医和卫生重要性以及同人类学而闻名。侧孢双孢子菌的巨大生物技术潜力已被证明，但对双翅目粘虫的研究仍然很少。该研究的目的是验证侧孢芽孢杆菌的死亡率NRS590 在同人类果蝇上，并在超微结构上表征其不同的细胞阶段。从垃圾箱中收集苍蝇，使菌落适应实验室条件。新幼虫的生物测定从以下阶段的细菌生长进行：营养（6 小时）、孢子囊（20 小时）和游离孢子（44 小时）。收集每一相的等分试样用于透射 (TEM) 和扫描电子显微镜 (SEM)。在孢子形成阶段，其中的校正死亡率为83.3，85.1，观察NRS590的有效性，和99％为大头金蝇，大头金putoria，和家蝇， 分别。在整个孢子表面上详细观察了副孢子体。尽管我们对这种细菌的了解不断增加，但仍有待确定导致在粘液双翅目上观察到的广泛昆虫致病活性的真正毒力因素。因此，本研究可为改进对环境和生物有效、安全的微生物控制技术提供补贴。
更新日期：2021-07-30
中文翻译：
侧孢短芽孢杆菌的超微结构和致病性对正嗜性粘液双翅目
侧孢短芽孢杆菌对几种昆虫具有致病潜力，其广泛的生物活性与多种菌株特异性分子有关。为了避免使用合成杀虫剂，以及防治害虫的需要，微生物防治已被广泛使用。双翅类粘虫以其医学兽医和卫生重要性以及同人类学而闻名。侧孢双孢子菌的巨大生物技术潜力已被证明，但对双翅目粘虫的研究仍然很少。该研究的目的是验证侧孢芽孢杆菌的死亡率NRS590 在同人类果蝇上，并在超微结构上表征其不同的细胞阶段。从垃圾箱中收集苍蝇，使菌落适应实验室条件。新幼虫的生物测定从以下阶段的细菌生长进行：营养（6 小时）、孢子囊（20 小时）和游离孢子（44 小时）。收集每一相的等分试样用于透射 (TEM) 和扫描电子显微镜 (SEM)。在孢子形成阶段，其中的校正死亡率为83.3，85.1，观察NRS590的有效性，和99％为大头金蝇，大头金putoria，和家蝇， 分别。在整个孢子表面上详细观察了副孢子体。尽管我们对这种细菌的了解不断增加，但仍有待确定导致在粘液双翅目上观察到的广泛昆虫致病活性的真正毒力因素。因此，本研究可为改进对环境和生物有效、安全的微生物控制技术提供补贴。
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