In this study, we evaluated cardiomyogenic differentiation of electromechanically stimulated rat bone marrow-derived stem cells (rt-BMSCs) on an acellular bovine pericardium (aBP) and we looked at the functioning of this engineered patch in a rat myocardial infarct (MI) model. aBP was prepared using a detergent-based decellularization procedure followed by rt-BMSCs seeding, and electrical, mechanical, or electromechanical stimulations (3 millisecond pulses of 5 V cm⁻¹ at 1 Hz, 5% stretching) to enhance cardiomyogenic differentiation. Furthermore, the electromechanically stimulated patch was applied to the MI region over 3 weeks. After this period, the retrieved patch and infarct region were evaluated for the presence of calcification, inflammatory reaction (CD68), patch to host tissue cell migration, and structural sarcomere protein expressions. In conjunction with any sign of calcification, a higher number of BrdU-labelled cells, and a low level of CD68 positive cells were observed in the infarct region under electromechanically stimulated conditions compared with static conditions. More importantly, MHC, SAC, Troponin T, and N-cad positive cells were observed in both infarct region, and retrieved engineered patch after 3 weeks. In a clear alignment with other results, our developed acellular patch promoted the expression of cardiomyogenic differentiation factors under electromechanical stimulation. Our engineered patch showed a successful integration with the host tissue followed by the cell migration to the infarct region.

在这项研究中，我们评估了机电刺激的大鼠骨髓干细胞 (rt-BMSCs) 在无细胞牛心包 (aBP) 上的心肌分化，并研究了这种工程贴片在大鼠心肌梗塞 (MI) 模型中的功能. aBP 是使用基于洗涤剂的脱细胞程序制备的，随后是 rt-BMSCs 播种，以及电、机械或机电刺激（3 毫秒脉冲 5 V cm ^-1在 1 Hz，5% 拉伸）以增强心肌分化。此外，将机电刺激贴片应用于 MI 区域超过 3 周。在此期间之后，评估取回的贴片和梗塞区域是否存在钙化、炎症反应 (CD68)、贴片到宿主组织细胞迁移和结构肌节蛋白表达。与任何钙化迹象相结合，与静态条件相比，在机电刺激条件下，在梗塞区域观察到更多数量的 BrdU 标记细胞和低水平的 CD68 阳性细胞。更重要的是，在两个梗塞区域均观察到 MHC、SAC、肌钙蛋白 T 和 N-cad 阳性细胞，并在 3 周后回收工程贴片。与其他结果明显一致，我们开发的无细胞贴片在机电刺激下促进了心肌分化因子的表达。我们的工程贴片显示与宿主组织成功整合，然后细胞迁移到梗塞区域。