Diabetic foot ulcers (DFU) are a vascular complication of diabetes mellitus (DM). It has been confirmed that irisin is closely related to DM. However, the effect of irisin on DFU is obscure and needs further study. After human umbilical vein endothelial cell lines (HUVECs) were treated with different concentrations' irisin, normal glucose, high glucose (HG), HG plus irisin-high (H) or sh-Notch1, cell biological behaviors, LDH, and VEGFA were detected by cell function experiments. Apoptosis- and Notch pathway-related protein levels were evaluated by Western blot. Irisin has no cytotoxicity, and irisin-H elevated cell viability and inhibited apoptosis and LDH level in HG-induced HUVECs. Meanwhile, irisin-H restored HG-repressed migration and angiogenesis in HUVECs. Irisin-H inhibited apoptosis-related protein levels and promoted VEGFA and Notch pathway-related protein levels in HG-treated HUVECs. Additionally, sh-Notch1 reversed the protective effect of irisin-H in HG-treated HUVECs. Irisin restores HG-induced cell injury and angiogenesis in HUVECs by activating Notch pathway via Notch1.

中文翻译：

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Irisin 通过 Notch 受体 1 激活 Notch 通路，恢复高葡萄糖诱导的血管内皮细胞细胞损伤。


糖尿病足溃疡（DFU）是糖尿病（DM）的血管并发症。现已证实鸢尾素与DM密切相关。然而，鸢尾素对 DFU 的影响尚不清楚，需要进一步研究。人脐静脉内皮细胞系（HUVEC）经不同浓度irisin、正常葡萄糖、高葡萄糖（HG）、HG加irisin-high（H）或sh-Notch1处理后，检测细胞生物学行为、LDH和VEGFA通过细胞功能实验。通过蛋白质印迹评估细胞凋亡和Notch途径相关的蛋白水平。 Irisin 无细胞毒性，并且 irisin-H 可以提高 HG 诱导的 HUVEC 细胞活力并抑制细胞凋亡和 LDH 水平。同时，irisin-H 恢复了 HUVEC 中 HG 抑制的迁移和血管生成。在 HG 处理的 HUVEC 中，Irisin-H 抑制凋亡相关蛋白水平并促进 VEGFA 和 Notch 通路相关蛋白水平。此外，sh-Notch1 逆转了 irisin-H 在 HG 处理的 HUVEC 中的保护作用。 Irisin 通过 Notch1 激活 Notch 通路，恢复 HG 诱导的 HUVEC 细胞损伤和血管生成。