A prevalent type of bone tumor, osteosarcoma (OS) is prone to pulmonary metastasis, which results in a high relapse risk and poor prognosis for patients. The progression of OS is significantly associated with the expression of long non‑coding (lnc)RNA H19. To the best of our knowledge, however, the exact molecular mechanism of this lncRNA has not been fully investigated. The present study verified the effect of H19 on the proliferation and invasion of osteosarcoma cells via in vivo and in vitro experiments, including Cell Counting Kit‑8, western blot, reverse transcription‑quantitative PCR, wound healing and Transwell assays. H19 was found to be overexpressed in OS compared with corresponding normal adjacent tissue. In addition, H19 served as a competing endogenous ncRNA targeting microRNA‑29a‑3p and activating LIM and SH3 domain protein 1 and modulating the OS cell phenotype. The results of the present study may improve understanding of OS pathogenesis.

中文翻译：

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长非编码 RNA H19 通过与 miR-29a-3p 竞争性结合来调节骨肉瘤中 LASP1 的表达。


骨肉瘤（OS）是一种常见的骨肿瘤，容易发生肺转移，导致患者复发风险高、预后差。 OS 的进展与长非编码 (lnc)RNA H19 的表达显着相关。然而，据我们所知，这种 lncRNA 的确切分子机制尚未得到充分研究。本研究通过体内和体外实验，包括细胞计数试剂盒8、蛋白质印迹、逆转录定量PCR、伤口愈合和Transwell实验，验证了H19对骨肉瘤细胞增殖和侵袭的影响。发现与相应的正常邻近组织相比，H19 在 OS 中过度表达。此外，H19 作为一种竞争性内源性 ncRNA，靶向 microRNA-29a-3p，激活 LIM 和 SH3 结构域蛋白 1，并调节 OS 细胞表型。本研究的结果可能会增进对 OS 发病机制的了解。