The detection of allergen-specific IgE is of value for the diagnosis of children’'s milk allergy. However, its accuracy will interfere with the presence of high levels of specific IgG in the serum of children with milk allergy. To solve this problem, we established a light-initiated chemiluminescent assay (LICA) based on nanomicrospheres, which neutralized the interference of specific IgG by increasing the amount of antigen coated on the microspheres. The ability of this method to resist IgG interference was confirmed by adding extra specific IgG to the serum of allergic patients. Finally, the positive rate of allergen-specific IgE was increased to 85%, which was better than the indirect ELISA (70%), indicating that this method has certain advantages for the detection of specific IgE in children with milk allergy.

过敏原特异性IgE的检测对于儿童牛奶过敏的诊断具有价值。然而，它的准确性会干扰牛奶过敏儿童血清中高水平特异性 ​​IgG 的存在。为了解决这个问题，我们建立了一种基于纳米微球的光引发化学发光分析（LICA），通过增加包被在微球上的抗原量来中和特异性IgG的干扰。通过向过敏患者的血清中添加额外的特异性 IgG，证实了该方法抵抗 IgG 干扰的能力。最后，过敏原特异性IgE的阳性率提高到85%，优于间接ELISA（70%），说明该方法对牛奶过敏儿童特异性IgE的检测具有一定优势。