Improving the health and immunity of pets is a concern of the guardians, while maintaining the health of the animals directly impacts the owners' health and public health. The objective of this study was to produce a dog feed containing a microencapsulated phytogenic blend, as well as determine the impact of the additive on animal health and its intestinal microbiota. The composition included microencapsulated thymol, carvacrol, and cinnamaldehyde, at 300 mg/kg of feed. Ten male beagle dogs were divided into two groups, identified as follows: the control group (C; ingested the feed without the additive) and the treated group (T; consumed feed containing the phytogenic blend). The dogs received 300 g of feed/day divided into two meals. Greater neutrophil counts in group T and lymphocyte counts were observed at the end of the experiment, as well as levels of α2-globulins and γ-globulin, while β1 and β2-globulins levels were lower in group T. Serum nitrogen oxide levels were higher in group T. Levels of reactive oxygen species were lower in group T at days 30 and 45, unlike activity of glutathione peroxidase that was higher. We found less bacterial contamination in the feces of dogs in group T, i.e., total bacterial count, total coliform counts, and counts of Salmonella and Escherichia coli were lower on days 30 and 45. Phytogenic blend intake reduces bacterial counts in stool and improves antioxidant/oxidative status and immune responses.

提高宠物的健康和免疫力是监护人关心的问题，而保持动物的健康直接关系到主人的身体健康和公众健康。本研究的目的是生产一种含有微胶囊植物原混合物的狗饲料，并确定添加剂对动物健康及其肠道微生物群的影响。该组合物包括微囊化百里酚、香芹酚和肉桂醛，按 300 mg/kg 饲料摄入。十只雄性比格犬被分成两组，如下确定：对照组（C；摄入不含添加剂的饲料）和处理组（T；食用含有植物性混合物的饲料）。狗每天接受 300 克饲料，分为两餐。在实验结束时观察到 T 组中性粒细胞计数和淋巴细胞计数更高，以及 α2-球蛋白和 γ-球蛋白的水平，而 T 组的 β1 和 β2-球蛋白水平较低。T 组的血清氮氧化物水平较高。 T 组在第 30 天和45，不像谷胱甘肽过氧化物酶的活性更高。我们发现T组狗的粪便中细菌污染较少，即细菌总数、大肠菌总数和沙门氏菌和大肠杆菌在第 30 天和第 45 天较低。植物混合摄入量减少粪便中的细菌数量，并改善抗氧化/氧化状态和免疫反应。