Journal of Environmental Health Science and Engineering ( IF 3.0 ) Pub Date : 2021-07-30 , DOI: 10.1007/s40201-021-00683-0 Mohamed Wageed 1 , Gamal M El-Sherbiny 2 , Mohammed H Sharaf 2 , Saad A Moghannem 2 , Ahmed H Hamzawy 1
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Purpose
Environmental contamination with various pesticides accompanied by uncontrolled use contributes to severe ecological and health problems. Although extensive research was conducted on pesticides degradation, very few reports have demonstrated the degradation of mixed pesticides. Consequently, this study aimed to evaluate the removal efficacy of highly potent bacterial isolate for pesticide mixture under optimal culture conditions, followed by their application in milk.
Methods
Isolation and selection of bacterial isolates were performed from 40 milk samples by enrichment culture technique and were screened to obtain highly potent bacterial strain identified by 16 S rDNA analysis. The statistics-based experimental designs were applied to optimize the culture conditions towards the best degradation of pesticides mixture, followed by subsequent utilization in milk. The degradation ratio of pesticides was analyzed using gas chromatography-mass spectrometry.
Results
In this study, a bacterial strain S6A identified as Bacillus subtilis-mw1 efficiently eliminated environmental contaminants from different groups of pesticide residues. The statistical optimization showcased optimum settings that accomplished the highest pesticide mixture degradation (61.59 %). The application experiment manifested that degradation of pesticide mixtures of sterile milk (STM) was relatively faster than non-sterile milk (NSTM).
Conclusions
The obtained results assist in eliminating environmental contamination with various groups of pesticide residues. Furthermore, it can be employed in reducing pesticide residues that cause milk contamination to increase safety and quality.
Graphical abstract
中文翻译：
响应面法增强单一菌株对十五种农药混合物的去除效果及其在原奶中的应用
目的
各种农药的环境污染以及不受控制的使用会导致严重的生态和健康问题。尽管对农药降解进行了广泛的研究，但很少有报道证明混合农药的降解。因此，本研究旨在评估高效细菌分离物在最佳培养条件下对农药混合物的去除效果，然后将其应用于牛奶。
方法
采用富集培养技术从40份牛奶样品中分离和筛选分离菌，筛选得到16 S rDNA分析鉴定的高效菌株。应用基于统计的实验设计来优化培养条件，以实现农药混合物的最佳降解，然后在牛奶中使用。采用气相色谱-质谱法分析农药的降解率。
结果
在这项研究中，被鉴定为枯草芽孢杆菌-mw1的菌株 S6A有效地消除了不同农药残留组中的环境污染物。统计优化展示了实现最高农药混合物降解 (61.59%) 的最佳设置。应用实验表明，无菌奶（STM）对农药混合物的降解速度相对比非无菌奶（NSTM）快。
结论
获得的结果有助于消除各种农药残留对环境的污染。此外，它还可用于减少导致牛奶污染的农药残留，从而提高安全性和质量。
图形概要
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