Shilajit has a longstanding use as an anti-aging and memory enhancing drug. It is known to have excellent anti-bacterial effects and is believed to be effective for cognitive enhancement, but is difficult to standardize because of the lack of quality control standards. This study, for the first time, proposes a quality control standard using a simultaneous analytical method for the drug’s multi-compound content using high-performance liquid chromatography-ultraviolet detection (HPLC-UV) as an aid for the internationalization of Mongolian Shilajit. Phenolic compounds 1-6 were isolated from Mongolian Shilajit extract using bioassay-guided isolation, and the isolated compounds were evaluated for cognitive-related anti-Alzheimer’s disease (AD) activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), β-site amyloid precursor protein-cleaving enzyme 1 (BACE1), and advanced glycation end-product (AGE) formation assays. The isolated compounds showed good effects for each activity. In addition, the isolated compounds were successfully quantified using a validated quantitative HPLC analysis method. As a result, the isolated compounds were suggested as standard marker compounds for Mongolian Shilajit. Also, we proved that the original material of Mongolian Shilajit is a lichen named Xanthoparmelia somloensis (Gyel.) Hale using HPLC-UV, ultra-high-performance liquid chromatography-electrospray ionization/hybrid linear trap-quadruple-orbitrap-high-resolution mass spectrometry (UHPLC-ESI/LTQ-HRMS).

Shilajit 长期以来一直用作抗衰老和增强记忆力的药物。众所周知，它具有极好的抗菌作用，被认为对认知增强有效，但由于缺乏质量控制标准，难以标准化。本研究首次提出了使用高效液相色谱-紫外检测（HPLC-UV）同时分析药物多化合物含量的质量控制标准，作为蒙古什拉吉国际化的辅助手段。酚类化合物1-6使用生物测定指导的分离从蒙古 Shilajit 提取物中分离出来，并使用 1,1-二苯基-2-苦基肼 (DPPH) 自由基清除剂乙酰胆碱酯酶评估分离的化合物的认知相关抗阿尔茨海默病 (AD) 活性。 AChE)、丁酰胆碱酯酶 (BChE)、β 位淀粉样前体蛋白裂解酶 1 (BACE1) 和晚期糖基化终产物 (AGE) 形成分析。分离出的化合物对各项活性均表现出良好的效果。此外，使用经过验证的定量 HPLC 分析方法成功地对分离的化合物进行了定量。结果，建议将分离的化合物作为蒙古Shilajit 的标准标记化合物。此外，我们证明了蒙古Shilajit的原始材料是一种名为Xanthoparmelia somloensi的地衣s (Gyel.) Hale 使用 HPLC-UV、超高效液相色谱-电喷雾电离/混合线性阱-四极杆-轨道阱-高分辨率质谱 (UHPLC-ESI/LTQ-HRMS)。