Evidence for Systemic Reactive Oxygen Species in UVB-mediated Microvesicle Formation,Photochemistry and Photobiology

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Evidence for Systemic Reactive Oxygen Species in UVB-mediated Microvesicle Formation
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2021-07-29 , DOI: 10.1111/php.13494
Cameron L McGlone ₁ , Lea Christian ₁ , Benjamin Schmeusser ₁ , Langni Liu ₁ , Charles E Chalfant _{2,

3} , Daniel J Stephensen ₂ , Catherine M Sherwin _{1,

4} , Christine M Rapp ₁ , Zafer Sattouf ₁ , Craig A Rohan _{1,

5} , Connor Morris ₁ , Yanfang Chen ₁ , Jeffrey B Travers _{1,

5,

6}

Affiliation

Recent studies have implicated subcellular microvesicle particles (MVP) in the ability of ultraviolet B radiation to exert both local and systemic effects. Indeed, UVB generates MVP (UVB-MVP) in human skin and systemically following phototherapy. The current studies were designed to test the hypothesis that the ability of UVB to generate MVP was dependent upon reactive oxygen species (ROS). To that end, we tested urine samples from subjects undergoing UVB phototherapy for the presence of isoprostanes as well as the oxidized guanosine derivative 8OHdG. We also conducted a clinical study in which volar forearms of subjects were treated with localized UVB and erythema/MVP measured. The same cohort was then treated with 7 days of vitamin C (2 g day⁻¹) and vitamin E (1000 IU day⁻¹), and UVB-induced MVPs tested on the contralateral forearm. Urine specimens from subjects undergoing phototherapy were found to have increased levels of isoprostanes and 8OHdG, with maximal levels noted 8–16 h post-treatment. Treatment with antioxidant vitamins resulted in diminished UVB-generated skin MVP to baseline levels. These studies suggest that whole-body UVB generates a systemic pro-oxidative response, and that antioxidants can attenuate localized skin UVB-MVPs.

中文翻译：

UVB 介导的微泡形成中全身活性氧的证据

最近的研究表明，亚细胞微泡颗粒 (MVP) 与紫外线 B 辐射发挥局部和全身效应的能力有关。事实上，UVB 在人体皮肤中产生 MVP (UVB-MVP)，并在光疗后全身产生。目前的研究旨在检验 UVB 产生 MVP 的能力取决于活性氧 (ROS) 的假设。为此，我们测试了接受 UVB 光疗的受试者的尿液样本中是否存在异前列腺素以及氧化鸟苷衍生物 8OHdG。我们还进行了一项临床研究，其中受试者的掌侧前臂接受局部 UVB 治疗并测量红斑/MVP。然后，同一队列接受 7 天的维生素 C（2 g 天^-1 ）和维生素 E（1000 IU 天^-1 ）治疗，并在对侧前臂上测试 UVB 诱导的 MVP。研究发现，接受光疗的受试者的尿液样本中异前列烷和 8OHdG 的水平有所增加，治疗后 8-16 小时达到最高水平。使用抗氧化维生素进行治疗后，UVB 产生的皮肤 MVP 降低至基线水平。这些研究表明，全身 UVB 会产生全身性促氧化反应，而抗氧化剂可以减弱局部皮肤 UVB-MVP。

更新日期：2021-07-29

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