Abstract

Aim

In this study, it was aimed to investigate the effect of coenzyme Q10 (CoQ10) on cisplatin-induced oxidative retinal damage in rats biochemically and histopathologically.

Materials and methods

Thirty male Wistar albino rats were divided into 3 groups randomly: untreated control (C group), only 2.5 mg/kg cisplatin daily administrated group for 2 weeks (CP group), 2.5 mg/kg cisplatin + 20 mg/kg orally CoQ10 daily administrated group for 2 weeks (CoQC group). At the end of experimental period, blood samples obtained before sacrification for the biochemical examination of serum malondialdehyde (MDA), total glutathione (tGSH), total oxidant system (TOS), total antioxidant systemic (TAS) levels and after eyes were removed for examined histopathology.

Results

As a result of our study, severe histopathological damage was detected in the retinal tissue of the cisplatin group with serum malondialdehyde (MDA) and total oxidant system (TOS) levels were high and total glutathione (tGSH) and total antioxidant systemic (TAS) levels were low. However, it was observed that the histopathological damage associated with cisplatin was decreased in the retinal tissue of the CoQ10 group, which inhibited the increase in blood serum MDA/TOS levels and decrease in tGSH/TAS levels.

Conclusion

The biochemical and histopathological results of our study were compatible with each other, so we concluded that the damage to the rat retinal tissue caused by cisplatin may be reversible with coenzyme.

中文翻译：

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辅酶Q10对顺铂诱导的大鼠氧化性视网膜损伤的影响

摘要

目标

本研究旨在从生化和组织病理学角度研究辅酶 Q10 (CoQ10) 对顺铂诱导的大鼠氧化性视网膜损伤的影响。

材料和方法

30 只雄性 Wistar 白化病大鼠随机分为 3 组：未治疗的对照组（C 组）、仅 2.5 mg/kg 顺铂每日给药组 2 周（CP 组）、2.5 mg/kg 顺铂 + 20 mg/kg 口服 CoQ10 每日给药组 2 周（CoQC 组）。在实验期结束时，取血样在处死前采集血清丙二醛（MDA）、总谷胱甘肽（tGSH）、总氧化系统（TOS）、总抗氧化系统（TAS）水平和眼球后进行生化检查。组织病理学。

结果

作为我们研究的结果，顺铂组的视网膜组织中检测到严重的组织病理学损伤，血清丙二醛（MDA）和总氧化系统（TOS）水平很高，总谷胱甘肽（tGSH）和总抗氧化系统（TAS）水平低。然而，观察到辅酶Q10组视网膜组织中与顺铂相关的组织病理学损伤减少，从而抑制了血清MDA/TOS水平的升高和tGSH/TAS水平的降低。

结论

我们研究的生化和组织病理学结果相互兼容，因此我们得出结论，顺铂对大鼠视网膜组织的损伤可能是辅酶可逆的。