Abstract

Diclofenac is one of the most prescribed non-steroidal anti-inflammatory drugs (NSAIDs) for acute and chronic inflammatory conditions. Taking into consideration the extensive use of diclofenac, the crucial role of inflammation in tumorigenesis and data on the effects of NSAIDs on cancer cell lines, we investigated the cytotoxic potential of diclofenac on a panel of human cell lines originating from breast (MCF-7), cervical (HeLa) and colorectal cancer (HT-29). The cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye reduction assay and the half maximal inhibitory concentrations (IC₅₀) were determined. Diclofenac had higher potency against MCF-7 and HT-29 than against HeLa. The cellular and nuclear changes were examined by fluorescent microscopy using 4′,6′-diamino-2-phenylindole (DAPI) stain and acridine orange/ethidium bromide (AO/EB). The wound-healing scratch assay showed significant reduction in the migration capacity of all tested cancer cell lines. The observed antineoplastic activity implies that the anticancer potential of NSAIDs and diclofenac, in particular, necessitates further investigation.

摘要

双氯芬酸是治疗急性和慢性炎症的处方最多的非甾体抗炎药 (NSAID) 之一。考虑到双氯芬酸的广泛使用、炎症在肿瘤发生中的关键作用以及 NSAIDs 对癌细胞系影响的数据，我们研究了双氯芬酸对一组源自乳腺的人类细胞系 (MCF-7) 的细胞毒性潜力、宫颈癌 (HeLa) 和结肠直肠癌 (HT-29)。使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 染料还原试验和半数最大抑制浓度 (IC ₅₀) 确定。双氯芬酸对 MCF-7 和 HT-29 的效力高于对 HeLa 的效力。使用 4',6'-二氨基-2-苯基吲哚 (DAPI) 染色和吖啶橙/溴化乙锭 (AO/EB) 通过荧光显微镜检查细胞和核变化。伤口愈合划痕试验显示所有测试癌细胞系的迁移能力显着降低。观察到的抗肿瘤活性意味着 NSAIDs 和双氯芬酸的抗癌潜力尤其需要进一步研究。