It has been reported that ACBD3 is closely related to the malignant process of cells, but its role in gastric cancer has not been elucidated. This study aims to investigate the expression and function of ACBD3 in human gastric cancer. The Cancer Genome Atlas (TCGA) database were selected to analyze mRNA levels of ACBD3 in gastric cancer tissues and normal gastric epithelial tissues. qPCR and Western blot were conducted to detect the expression of ACBD3 in two normal gastric epithelial cell lines and five gastric cancer cell lines which were cultured in our laboratory. To exclude differences in individual background between different patients, we further detected the expression of ACBD3 in 8 pairs of malignant/non-malignant clinical gastric tissues. Through the establishment of stable cells, in vitro cell experiments and in vivo xenotransplantation models in mice, the role of ACBD3 in the proliferation of gastric cancer cells has been further explored. AKT inhibitors were used to deeply explore the molecular regulation mechanism of ACBD3. The results showed that the elevated ACBD3 in gastric cancer tissue were positively correlated with the clinical grade and prognosis of gastric cancer. In terms of molecular function, we found that ACBD3 can enhance the production and growth of gastric cancer cells. At the same time, the activation of AKT kinase played an important role in ACBD3's promotion of G1-to-S transition. The experiments generally indicate that ACBD3 is expected to become a potential diagnostic molecule or therapeutic target for gastric cancer.

已有报道ACBD3与细胞的恶性过程密切相关，但其在胃癌中的作用尚未阐明。本研究旨在探讨ACBD3在人胃癌中的表达及功能。选择癌症基因组图谱（TCGA）数据库分析胃癌组织和正常胃上皮组织中ACBD3的mRNA水平。采用qPCR和Western blot检测本实验室培养的2个正常胃上皮细胞系和5个胃癌细胞系中ACBD3的表达。为了排除不同患者个体背景的差异，我们进一步检测了8对恶性/非恶性临床胃组织中ACBD3的表达。通过建立稳定的细胞，通过小鼠体外细胞实验和体内异种移植模型，进一步探讨了ACBD3在胃癌细胞增殖中的作用。AKT抑制剂用于深入探索ACBD3的分子调控机制。结果表明，胃癌组织中ACBD3升高与胃癌的临床分级和预后呈正相关。在分子功能方面，我们发现ACBD3可以增强胃癌细胞的产生和生长。同时，AKT激酶的激活在ACBD3促进G1-to-S转变中起重要作用。实验普遍表明，ACBD3有望成为胃癌的潜在诊断分子或治疗靶点。