Recent progress in three-dimensional (3D) cell culture systems has attracted much attention in the fields of basic life science and drug development. Newly established methods include 3D co-culture, spheroid culture, and organoid culture; these methods enable more human tissue-like culture and have largely replaced traditional two-dimensional (2D) monolayer culture. By combining 3D culture methods with high-content imaging analysis, it is possible to obtain diverse and convincing data even during initial screening (which requires rapid and easy operating procedures). Until recently, 3D culture methods were considered expensive, time-consuming, complex, and unstable. However, by exploiting the self-assembling nature of cells and adding several technical improvements, we have developed several phenotypic screenings aimed at discovering anticancer compounds.

三维 (3D) 细胞培养系统的最新进展引起了基础生命科学和药物开发领域的广泛关注。新建立的方法包括 3D 共培养、球体培养和类器官培养；这些方法使更多的人体组织样培养成为可能，并在很大程度上取代了传统的二维 (2D) 单层培养。通过将 3D 培养方法与高内涵成像分析相结合，即使在初始筛选期间（这需要快速简便的操作程序）也可以获得多样化和令人信服的数据。直到最近，3D 培养方法还被认为是昂贵、耗时、复杂且不稳定的。然而，通过利用细胞的自组装特性并增加多项技术改进，