Early malaria diagnosis and its profiling require the development of new sensing platforms enabling rapid and early analysis of parasites in blood or saliva, aside the widespread rapid diagnostic tests (RDTs). This study shows the performance of a cost-effective optical fiber-based solution to target the presence of Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Unclad multimode optical fiber probes are coated with a thin gold film to excite Surface Plasmon Resonance (SPR) yielding high sensitivity to bio-interactions between targets and bioreceptors grafted on the metal surface. Their performances are presented in laboratory conditions using PBS spiked with growing concentrations of purified target proteins and within in vitro cultures. Two probe configurations are studied through label-free detection and amplification using secondary antibodies to show the possibility to lower the intrisic limit of detection. As malaria hits millions of people worldwide, the improvement and multiplexing of this optical fiber technique can be of great interest, especially for a future purpose of using multiple receptors on the fiber surface or several coated-nanoparticles as amplifiers.

中文翻译：

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使用等离子体光极检测 PfHRP2：性能分析

除了广泛的快速诊断测试 (RDT) 之外，早期的疟疾诊断及其分析需要开发新的传感平台，以便对血液或唾液中的寄生虫进行快速和早期分析。这项研究显示了一种基于成本效益的光纤解决方案的性能，该解决方案针对恶性疟原虫富含组氨酸的蛋白质 2 (PfHRP2) 的存在。未包覆的多模光纤探头涂有一层薄金膜，以激发表面等离子体共振 (SPR)，从而对目标和接枝在金属表面上的生物受体之间的生物相互作用产生高灵敏度。它们的性能在实验室条件下使用掺有浓度不断增加的纯化目标蛋白的 PBS 和体外培养物进行展示。通过无标记检测和使用二抗扩增来研究两种探针配置，以显示降低检测限的可能性。随着疟疾侵袭全球数百万人，这种光纤技术的改进和多路复用可能会引起人们极大的兴趣，尤其是在未来使用光纤表面上的多个受体或几个涂层纳米粒子作为放大器的目的时。