Abstract

A sensitive electrochemical matrix based on In₂O₅Sn nanoparticles modified fluorinated tin oxide (SnO₂:F) conducting glass was reported. Besides, urease (Urs) was physically immobilized over the In₂O₅Sn/SnO₂:F matrix surface for fast and simple trace urea quantification with a novel mechanism. Initially, the qualities of constructed In₂O₅Sn nanoparticles were considered by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The nanoparticles reveal exceptional effects in the Urs redox reaction on Urs/In₂O₅Sn/SnO₂:F biosensor. Therefore, urea concentration was obtained directly by electrochemical techniques with high sensitivity and excellent selectivity. The fabricated biosensor affords a unique response over a wide concentration range of 5–175 mg/dL for urea with the low detection limit (3 mg/dL), fast response time (less than 3 s) and relatively long life span (40 days). Stability with verification of the suitability of the modified Urs/In₂O₅Sn/SnO₂:F biosensor in the analysis of blood serum was assessed. The results obtained were in agreement with those attained from the independent standard method.

中文翻译：

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基于脲酶/In2O5Sn纳米涂层氟化SnO2的灵敏生物传感器用于检测血清中的尿素

摘要

报道了一种基于 In ₂ O ₅ Sn 纳米颗粒改性氟化氧化锡 ( SnO ₂ :F ) 导电玻璃的灵敏电化学基质。此外，脲酶 ( Urs ) 物理固定在 In ₂ O ₅ Sn/SnO ₂ :F 基质表面上，以一种新颖的机制快速简单地对痕量尿素进行定量。最初，构建的 In ₂ O ₅ Sn 纳米粒子的质量是通过扫描电子显微镜和能量色散 X 射线光谱法来考虑的。纳米粒子揭示了 Urs 氧化还原反应对 Urs/In ₂ O _{5 的}特殊影响Sn/SnO ₂ :F 生物传感器。因此，尿素浓度是通过电化学技术直接获得的，具有高灵敏度和优异的选择性。制造的生物传感器在 5-175 mg/dL 的宽浓度范围内对尿素提供独特的响应，具有低检测限 (3 mg/dL)、快速响应时间（小于 3 秒）和相对较长的寿命（40 天） ）。通过验证改进的 Urs/In ₂ O ₅ Sn/SnO ₂ :F 生物传感器在血清分析中的适用性来评估稳定性。获得的结果与从独立标准方法获得的结果一致。