Background. Parkinson’s disease (PD) is a common neurological degenerative disease that cannot be completely cured, although drugs can improve or alleviate its symptoms. Optogenetic technology, which stimulates or inhibits neurons with excellent spatial and temporal resolution, provides a new idea and approach for the precise treatment of Parkinson’s disease. However, the neural mechanism of photogenetic regulation remains unclear. Objective. In this paper, we want to study the nonlinear features of EEG signals in the striatum and globus pallidus through optogenetic stimulation of the substantia nigra compact part. Methods. Rotenone was injected stereotactically into the substantia nigra compact area and ventral tegmental area of SD rats to construct rotenone-treated rats. Then, for the optogenetic manipulation, we injected adeno-associated virus expressing channelrhodopsin to stimulate the globus pallidus and the striatum with a 1 mW blue light and collected LFP signals before, during, and after light stimulation. Finally, the collected LFP signals were analyzed by using nonlinear dynamic algorithms. Results. After observing the behavior and brain morphology, 16 models were finally determined to be successful. LFP results showed that approximate entropy and fractal dimension of rats in the control group were significantly greater than those in the experimental group after light treatment (). The LFP nonlinear features in the globus pallidus and striatum of rotenone-treated rats showed significant statistical differences before and after light stimulation (). Conclusion. Optogenetic technology can regulate the characteristic value of LFP signals in rotenone-treated rats to a certain extent. Approximate entropy and fractal dimension algorithm can be used as an effective index to study LFP changes in rotenone-treated rats.

中文翻译：

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光遗传学技术对鱼藤酮处理大鼠基底节核LFP的调控作用研究

背景。帕金森病 (PD) 是一种常见的神经退行性疾病，虽然药物可以改善或缓解其症状，但无法完全治愈。光遗传学技术以优异的时空分辨率刺激或抑制神经元，为帕金森病的精准治疗提供了新的思路和途径。然而，光遗传调控的神经机制仍不清楚。客观。在本文中，我们想通过光遗传学刺激黑质致密部分来研究纹状体和苍白球脑电信号的非线性特征。方法. 将鱼藤酮立体定向注射到SD大鼠黑质致密区和腹侧被盖区，构建鱼藤酮处理大鼠。然后，对于光遗传学操作，我们注射了表达通道视紫红质的腺相关病毒，以用 1 mW 蓝光刺激苍白球和纹状体，并在光刺激之前、期间和之后收集 LFP 信号。最后，使用非线性动态算法对收集到的 LFP 信号进行分析。结果。观察行为和大脑形态后，最终确定16个模型成功。LFP结果显示，光照处理后，对照组大鼠的近似熵和分形维数显着大于实验组（）。鱼藤酮处理的大鼠苍白球和纹状体的 LFP 非线性特征在光刺激前后表现出显着的统计学差异。）。 结论。光遗传学技术可以在一定程度上调节鱼藤酮处理大鼠LFP信号的特征值。近似熵和分形维数算法可作为研究鱼藤酮处理大鼠 LFP 变化的有效指标。