Reproducible Breath Metabolite Changes in Children with SARS-CoV-2 Infection,ACS Infectious Diseases

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Reproducible Breath Metabolite Changes in Children with SARS-CoV-2 Infection
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2021-07-28 , DOI: 10.1021/acsinfecdis.1c00248
Amalia Z Berna ₁ , Elikplim H Akaho ₁ , Rebecca M Harris _{1,

2,

3} , Morgan Congdon _{1,

3} , Emilie Korn _{1,

3} , Samuel Neher _{1,

3} , Mirna M'Farrej _{1,

3} , Julianne Burns _{1,

3} , Audrey R Odom John _{1,

3}

Affiliation

SARS-CoV-2 infection is diagnosed through detection of specific viral nucleic acid or antigens from respiratory samples. These techniques are relatively expensive, slow, and susceptible to false-negative results. A rapid noninvasive method to detect infection would be highly advantageous. Compelling evidence from canine biosensors and studies of adults with COVID-19 suggests that infection reproducibly alters human volatile organic compound (VOC) profiles. To determine whether pediatric infection is associated with VOC changes, we enrolled SARS-CoV-2 infected and uninfected children admitted to a major pediatric academic medical center. Breath samples were collected from children and analyzed through state-of-the-art GCxGC-ToFMS. Isolated features included 84 targeted VOCs. Candidate biomarkers that were correlated with infection status were subsequently validated in a second, independent cohort of children. We thus find that six volatile organic compounds are significantly and reproducibly increased in the breath of SARS-CoV-2 infected children. Three aldehydes (octanal, nonanal, and heptanal) drew special attention, as aldehydes are also elevated in the breath of adults with COVID-19. Together, these biomarkers demonstrate high accuracy for distinguishing pediatric SARS-CoV-2 infection and support the ongoing development of novel breath-based diagnostics.

中文翻译：

SARS-CoV-2 感染儿童的可重现呼吸代谢物变化

SARS-CoV-2 感染是通过检测呼吸道样本中的特定病毒核酸或抗原来诊断的。这些技术相对昂贵、速度慢并且容易出现假阴性结果。检测感染的快速非侵入性方法将是非常有利的。来自犬类生物传感器的有力证据和对 COVID-19 成年人的研究表明，感染可重复地改变人类挥发性有机化合物 (VOC) 的分布。为了确定儿科感染是否与 VOC 变化有关，我们招募了一家主要儿科学术医疗中心收治的 SARS-CoV-2 感染和未感染儿童。从儿童身上采集呼吸样本，并通过最先进的 GCxGC-ToFMS 进行分析。独立特征包括 84 种目标 VOC。随后在第二个独立的儿童队列中验证了与感染状态相关的候选生物标志物。因此，我们发现在 SARS-CoV-2 感染儿童的呼吸中，六种挥发性有机化合物显着且可重复地增加。三种醛（辛醛、壬醛和庚醛）引起了特别关注，因为醛在 COVID-19 成人患者的呼吸中也会升高。总之，这些生物标志物在区分儿科 SARS-CoV-2 感染方面表现出很高的准确性，并支持基于呼吸的新型诊断方法的持续发展。和庚醛）引起了特别关注，因为醛类在 COVID-19 成人患者的呼吸中也会升高。总之，这些生物标志物在区分儿科 SARS-CoV-2 感染方面表现出很高的准确性，并支持基于呼吸的新型诊断方法的持续发展。和庚醛）引起了特别关注，因为醛类在 COVID-19 成人患者的呼吸中也会升高。总之，这些生物标志物在区分儿科 SARS-CoV-2 感染方面表现出很高的准确性，并支持基于呼吸的新型诊断方法的持续发展。

更新日期：2021-09-10

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