Gold Nanoparticles as Carriers for Functional RNA Nanostructures,Bioconjugate Chemistry

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Gold Nanoparticles as Carriers for Functional RNA Nanostructures
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2021-07-29 , DOI: 10.1021/acs.bioconjchem.1c00211
Anna Graczyk ₁ , Roza Pawlowska ₁ , Arkadiusz Chworos ₁

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Conjugates of gold nanoparticles and ribonucleic acid are particularly interesting for biological applications to serve as therapeutics or biosensors. In this paper we present, for the first time, a conjugate of gold nanoparticles and structural RNA (tectoRNA), which serves as a tool for gene expression regulation. The tectoRNA trimer was modified to facilitate the introduction of a thiol linker, which aids the formation of stable RNA:AuNP conjugates. We demonstrated that these complexes can penetrate cells, which were observed in TEM analysis and are effective in gene expression regulation evident in GFP expression studies with fluorescence methods. The presented compounds have the potential to become a new generation of therapeutics that utilize the power of self-assembling, biologically active RNAs and gold nanoparticles, with their diagnostically useful optical properties and biocompatibility advantages.

中文翻译：

金纳米粒子作为功能性 RNA 纳米结构的载体

金纳米粒子和核糖核酸的结合物对于作为治疗剂或生物传感器的生物应用特别有趣。在本文中，我们首次介绍了金纳米粒子和结构 RNA (tectoRNA) 的结合物，它可作为基因表达调控的工具。tectoRNA 三聚体经过修饰以促进硫醇接头的引入，这有助于形成稳定的 RNA:AuNP 偶联物。我们证明这些复合物可以穿透细胞，这在 TEM 分析中观察到，并且在使用荧光方法的 GFP 表达研究中明显的基因表达调控中是有效的。所提出的化合物有可能成为利用自组装、生物活性 RNA 和金纳米粒子的力量的新一代疗法，

更新日期：2021-08-19

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