Complete vascular occlusion to distant tissue prior to an ischemic cardiac event can provide significant cardioprotection via remote ischemic preconditioning (RIPC). Despite understanding its mechanistic basis, its translation to clinical practice has been unsuccessful, likely secondary to the inherent impossibility of predicting (and therefore preconditioning) an ischemic event, as well as the discomfort that is associated with traditional, fully occlusive RIPC stimuli. Our laboratory has previously shown that non-occlusive banding (NOB) via wrapping of a leather band (similar to a traditional Jewish ritual) can elicit an RIPC response in healthy human subjects. This study sought to further the pain-mediated aspect of this observation in a mouse model of NOB with healthy mice that were exposed to treatment with and without lidocaine to inhibit pain sensation prior to ischemia/reperfusion injury. We demonstrated that NOB downregulates key inflammatory markers resulting in a preconditioning response that is partially mediated via pain sensation.

在缺血性心脏事件之前对远处组织进行完全血管闭塞可以通过远程缺血预处理（RIPC）提供显着的心脏保护作用。尽管了解其机制基础，但其向临床实践的转化并不成功，这可能是由于固有的不可能预测（因此预处理）缺血事件以及与传统的完全闭塞 RIPC 刺激相关的不适。我们的实验室之前已经表明，通过包裹皮革带（类似于传统的犹太仪式）的非闭塞带（NOB）可以在健康人类受试者中引起 RIPC 反应。本研究试图在 NOB 小鼠模型中进一步研究疼痛介导的方面，其中健康小鼠在缺血/再灌注损伤之前接受或不接受利多卡因治疗以抑制疼痛感。我们证明 NOB 下调关键炎症标志物，导致部分通过痛觉介导的预处理反应。