Silver nanoparticles have attracted much interest from scientists to develop nanosilver-based disinfectant products due to their unique properties of high antimicrobial activity. This study focused on biosynthesis, characterization, antimicrobial and antibiofilm effects of silver nanoparticles against vegetative and starved Shigella strains. The silver nanoparticles were synthesized using the yeast Yarrowia lipolytica and characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial and antibiofilm activities of silver nanoparticles were tested against the growth of vegetative and starved Shigella strains. After the addition of silver nitrate solution to the supernatant of Y. lipolytica, we noticed the appearance of a brown-black coloration that suggested the formation of silver nanoparticles. The presence of silver nanoparticles was manifested by a maximum absorption in the ultraviolet–visible range, precisely at the wavelength 420nm. The crystalline nature and the stability of silver nanoparticles were confirmed, respectively, by XRD and FTIR analysis. The antibacterial activity of silver nanoparticles showed significant toxicity on Shigella strains indicating that the starved cells were more sensitive to treatment with silver nanoparticles than vegetative cells. Surprisingly, the biofilm formation had not been inhibited by silver nanoparticles for both vegetative and starved cells. In conclusion, a new class of nanosilver containing disinfectant nanoproducts will be promising for advanced environmental treatments including air disinfection, water disinfection, surface disinfection and personal hygiene that will help to prevent the further outbreak of diseases.

中文翻译：

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解脂耶氏酵母产生的银纳米颗粒对营养和饥饿志贺氏菌的抗菌和抗菌作用

银纳米粒子由于其独特的高抗菌活性特性，引起了科学家们开发纳米银消毒剂产品的极大兴趣。本研究的重点是银纳米粒子对植物和饥饿的生物合成、表征、抗菌和抗生物膜作用志贺氏菌菌株。使用酵母合成银纳米粒子解脂耶氏酵母并通过紫外 - 可见光谱，X射线衍射（XRD）和傅里叶变换红外（FTIR）光谱进行表征。银纳米粒子的抗微生物和抗生物膜活性针对营养和饥饿的生长进行了测试志贺氏菌菌株。上清液中加入硝酸银溶液后解脂耶氏酵母，我们注意到棕黑色的出现表明银纳米粒子的形成。银纳米粒子的存在表现为紫外-可见范围内的最大吸收，恰好在波长 420纳米。通过XRD和FTIR分析分别证实了银纳米粒子的结晶性质和稳定性。银纳米粒子的抗菌活性显示出显着的毒性志贺氏菌菌株表明饥饿细胞对银纳米粒子的处理比营养细胞更敏感。令人惊讶的是，对于营养细胞和饥饿细胞，银纳米粒子都没有抑制生物膜的形成。总之，一种新型的含有纳米银消毒剂的纳米产品将有望用于先进的环境处理，包括空气消毒、水消毒、表面消毒和个人卫生，这将有助于防止疾病的进一步爆发。