Silver oxide Nanoparticles (SO-NP) exhibit excellent light absorbing, semi conducting properties and hence are employed in a wide range of applications such as catalyst, biosensors, and in fuel cells. Green synthesis of nanoparticles using different microorganisms is widely accepted since this method is in expensive and eco-friendly. Nanoparticles synthesized by this route are smaller in size, highly stable, show high reactivity and stability. In this context, biofabrication of Silver Oxide Nanoparticles (SO-NP) by autolysate of Pseudomonas mendocina PM1 has been evaluated. Synthesis of SO-NP was observed, when autolysate of P. mendocina PM1 was incubated with 0.5 mM AgNO3 in dark for 24 h. Synthesis of SO-NP was confirmed by UV-Vis analysis. SO-NP was further confirmed by Transmission Electron Microscopy (TEM) and X-ray Diffraction (XRD) which confirmed presence of SO-NP. XRD revealed that SO-NP were of the type Ag3O4. FTIR analysis indicated that peptides were involved in the reduction and stability of SO-NP. SO-NP’s showed potent anti-microbial/ anti-biofilm activity against common pathogenic/non-pathogenic bacteria. This is the first report of synthesis of SO-NP by P. mendocina PM1.

中文翻译：

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Pseudomonas mendocina PM1 自溶产物氧化银纳米颗粒 (SO-NP) 的生物制造，及其抗菌/抗生物膜潜力的评估

氧化银纳米颗粒 (SO-NP) 表现出优异的吸光性和半导电性，因此被广泛应用于催化剂、生物传感器和燃料电池等领域。使用不同微生物绿色合成纳米颗粒被广泛接受，因为这种方法昂贵且环保。通过该途径合成的纳米颗粒尺寸较小，稳定性高，表现出较高的反应活性和稳定性。在这种情况下，已经评估了通过门多克假单胞菌 PM1 的自溶产物对氧化银纳米颗粒 (SO-NP) 的生物制造。当 P. mendocina PM1 的自溶产物与 0.5 mM AgNO3 在黑暗中孵育 24 小时时，观察到 SO-NP 的合成。SO-NP 的合成通过 UV-Vis 分析得到证实。SO-NP 通过透射电子显微镜 (TEM) 和 X 射线衍射 (XRD) 进一步证实，这证实了 SO-NP 的存在。XRD 显示 SO-NP 是 Ag3O4 类型。FTIR 分析表明肽参与了 SO-NP 的还原和稳定性。SO-NP 对常见病原菌/非病原菌显示出有效的抗微生物/抗生物膜活性。这是 P. mendocina PM1 合成 SO-NP 的第一份报告。