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Detection of Serotonin, Melatonin, and Their Metabolites in Honey
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-07-28 , DOI: 10.1021/acsfoodscitech.1c00119 Tae-Kang Kim 1 , Venkatram R Atigadda 2 , Pawel Brzeminski 3 , Adrian Fabisiak 3 , Edith K Y Tang 4 , Robert C Tuckey 4 , Russel J Reiter 5 , Andrzej T Slominski 1
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-07-28 , DOI: 10.1021/acsfoodscitech.1c00119 Tae-Kang Kim 1 , Venkatram R Atigadda 2 , Pawel Brzeminski 3 , Adrian Fabisiak 3 , Edith K Y Tang 4 , Robert C Tuckey 4 , Russel J Reiter 5 , Andrzej T Slominski 1
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Melatonin and serotonin, products of tryptophan metabolism, are endogenous neurotransmitters and hormones. We have identified and quantified these metabolites in natural honey from Australia, USA, and Poland using a Xevo G2 XS qTof LC–MS. To help ensure correct product identification, some samples were prepurified by RP-HPLC based on the retention times of standards, prior to LC–MS. The concentrations of the metabolites of interest depended on the source of the honey. For Australian honey, levels for melatonin and 2-hydroxymelatonin were 0.91 and 0.68 ng/g, respectively. Melatonin was detected in one brand of US commercial honey at 0.48 ng/g, while a second brand contained serotonin at 88.2 ng/g. In Polish natural honey, 20.6 ng/g of serotonin and 40.8 ng/g of N-acetylserotonin (NAS) were detected, while in Polish commercial honey 25.9 ng/g of serotonin and 7.30 ng/g of NAS were present. We suggest that addictive and health-related properties of honey may be in part dependent on the presence of serotonin, melatonin, and their metabolites, and that these compounds may play a role in the colony activities of bees.
中文翻译：
蜂蜜中血清素、褪黑激素及其代谢物的检测
褪黑激素和血清素是色氨酸代谢的产物，是内源性神经递质和激素。我们使用 Xevo G2 XS qTof LC-MS 鉴定并量化了来自澳大利亚、美国和波兰的天然蜂蜜中的这些代谢物。为帮助确保正确的产品鉴定，一些样品在 LC-MS 之前根据标准的保留时间通过 RP-HPLC 进行了预纯化。目标代谢物的浓度取决于蜂蜜的来源。对于澳大利亚蜂蜜，褪黑激素和 2-羟基褪黑激素的含量分别为 0.91 和 0.68 ng/g。在一个品牌的美国商业蜂蜜中检测到褪黑激素含量为 0.48 ng/g，而第二个品牌的血清素含量为 88.2 ng/g。在波兰天然蜂蜜中，20.6 ng/g 的血清素和 40.8 ng/g 的N检测到-乙酰血清素 (NAS)，而在波兰商业蜂蜜中，血清素含量为 25.9 ng/g，NAS 含量为 7.30 ng/g。我们认为蜂蜜的成瘾性和与健康相关的特性可能部分取决于血清素、褪黑激素及其代谢物的存在，并且这些化合物可能在蜜蜂的蜂群活动中发挥作用。
更新日期：2021-08-20
中文翻译：
蜂蜜中血清素、褪黑激素及其代谢物的检测
褪黑激素和血清素是色氨酸代谢的产物，是内源性神经递质和激素。我们使用 Xevo G2 XS qTof LC-MS 鉴定并量化了来自澳大利亚、美国和波兰的天然蜂蜜中的这些代谢物。为帮助确保正确的产品鉴定，一些样品在 LC-MS 之前根据标准的保留时间通过 RP-HPLC 进行了预纯化。目标代谢物的浓度取决于蜂蜜的来源。对于澳大利亚蜂蜜，褪黑激素和 2-羟基褪黑激素的含量分别为 0.91 和 0.68 ng/g。在一个品牌的美国商业蜂蜜中检测到褪黑激素含量为 0.48 ng/g，而第二个品牌的血清素含量为 88.2 ng/g。在波兰天然蜂蜜中，20.6 ng/g 的血清素和 40.8 ng/g 的N检测到-乙酰血清素 (NAS)，而在波兰商业蜂蜜中，血清素含量为 25.9 ng/g，NAS 含量为 7.30 ng/g。我们认为蜂蜜的成瘾性和与健康相关的特性可能部分取决于血清素、褪黑激素及其代谢物的存在，并且这些化合物可能在蜜蜂的蜂群活动中发挥作用。
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