Bacterial nanocellulose (BNC) is a biocompatible material with a lot of potential. To make BNC commercially feasible, improvements in its production and surface qualities must be made. The in-situ fermentation and generation of BNC were investigated in this study by addition of different cellulosic substrates such as Avicel and carboxymethylcellulose (CMC) were performed using Komagataeibacter sp. SFCB22-18. The addition of cellulosic substrates improved BNC production by a maximum of about 5 times and slightly modified its structural properties. The changes in morphological and structural changes of BNC were investigated by using Fourier transform-infrared spectroscopy (FT-IR) spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). Furthermore, a type-A cellulose-binding protein, CtCBD3, was used in a novel biological analytic approach to measure the surface crystallinity of the BNC surface. Because Avicel and CMC may adhere to microfibrils during BNC synthesis or crystallization, cellulose-binding protein could be a useful tool for identifying the crystalline properties of BNC with high sensitivity.

中文翻译：

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将各种纤维素成分添加到细菌纳米纤维素中：表面质量和结晶特性的比较。

细菌纳米纤维素 (BNC) 是一种具有巨大潜力的生物相容性材料。为了使 BNC 在商业上可行，必须改进其生产和表面质量。本研究通过添加不同的纤维素底物（如 Avicel）和使用Komagataeibacter进行羧甲基纤维素 (CMC) 来研究 BNC 的原位发酵和生成sp。SFCB22-18。添加纤维素底物最多可将 BNC 产量提高约 5 倍，并略微改变其结构特性。通过使用傅里叶变换红外光谱 (FT-IR) 光谱、扫描电子显微镜 (SEM) 和 X 射线衍射 (XRD) 研究了 BNC 的形态和结构变化。此外，A 型纤维素结合蛋白 CtCBD3 被用于一种新的生物分析方法，以测量 BNC 表面的表面结晶度。由于 Avicel 和 CMC 在 BNC 合成或结晶过程中可能会粘附在微纤维上，因此纤维素结合蛋白可能是一种有用的工具，可以高灵敏度地识别 BNC 的结晶特性。