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Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., isolated from soil.
Journal of Microbiology and Biotechnology ( IF 2.8 ) Pub Date : 2021-07-21 , DOI: 10.4014/jmb.2105.05023
Shehzad Abid Khan 1 , Hyung Min Kim 1 , Ju Hye Baek 1 , Hye Su Jung 1 , Che Ok Jeon 1
Affiliation  

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242T and B156T, were isolated from soil in South Korea. Cells of strain H242T were oxidase-positive and non-motile short rods, while those of strain B156T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C16:0, cyclo-C17:0, andsummed feature 3 (C16:1ω7c and/or C16:1ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242T and B156T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242T and B156T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242T and B156T was 24.6%. Strains H242T and B156T were most closely related to Ramlibacter ginsenosidimutans BXN5-27T and Ramlibacter monticola G-3-2T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242T and R. ginsenosidimutans and between strain B156T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242T and B156T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242T (=KACC 21667T =JCM 33922T) and B156T (=KACC 21665T =JCM 33920T), respectively.

中文翻译:

Ramlibacter terrae sp。十一月 和 Ramlibacter montanisoli sp。十一月,从土壤中分离出来。

从韩国土壤中分离出两种革兰氏阴性、过氧化氢酶阳性、严格好氧和白色菌落形成细菌,菌株 H242 T和 B156 T。菌株H242 T的细胞是氧化酶阳性和不能运动的短杆状细胞,而菌株B156 T的细胞是氧化酶阴性和不能运动的长杆状细胞。Ubiquinone-8 被确定为两种菌株中唯一的类异戊二烯醌。C 16:0、cyclo-C 17:0和求和特征 3(C 16:1 ω 7 c和/或 C 16:1 ω 6 c) 和磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油在这两种菌株中分别被鉴定为主要的细胞脂肪酸和极性脂质。菌株H242 T和B156 T的DNA G+C含量分别为69.4 mol%和69.3 mol%。基于 16S rRNA 和 92 个连锁核心基因序列的系统发育分析表明,菌株 H242 T和 B156 T形成了与其他Ramlibacter型菌株不同的系统发育谱系。菌株H242 T和B156 T之间的DNA-DNA杂交(DDH)值为24.6%。菌株 H242 T和 B156 TRamlibacter ginsenosidimutans BXN5-27 TRamlibacter monticola G-3-2 T分别具有 98.4% 和 98.6% 的 16S rRNA 基因序列相似性。菌株 H242 TR. ginsenosidimutans之间以及菌株 B156 TR. monticola之间的数字 DDH 值分别为 23.5% 和 26.1%。表型、化学分类学和分子分析表明菌株 H242 T和 B156 T代表 Ramlibacter 属的两个新物种,名称为Ramlibacter terrae sp。十一月 和Ramlibacter montanisolsp。十一月,分别提出。R. terraeR. montanisoli的模式菌株分别是 H242 T (=KACC 21667 T =JCM 33922 T ) 和 B156 T (=KACC 21665 T =JCM 33920 T )。
更新日期:2021-07-21
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