Modified Renshen Wumei decoction (MRWD), a famous traditional Chinese medicine, is widely used for treating persistent diarrhoea. However, the mechanism by which MRWD regulates diarrhoea remains unknown. Therefore, we examined the protective effects of MRWD on intestinal barrier integrity in a diarrhoea model. In total, 48 male rats were randomly distributed to four treatment groups: the blank group (CK group), model group (MC group), Medilac-Vita group (MV group) and Chinese herb group (MRWD group). After a 10-day experiment, serum and colon samples were assessed. The diarrhoea index, pathological examination findings and change in D-lactate and diamine oxidase (DAO) contents illustrated that the induction of diarrhoea caused intestinal injury, which was ameliorated by MV and MRWD infusion. Metabolomics analysis identified several metabolites in the serum. Some critical metabolites, such as phosphoric acid, taurine, cortisone, leukotriene B4 and calcitriol, were found to be significantly elevated by MRWD infusion. Importantly, these differences correlated with mineral absorption and metabolism and peroxisome proliferator activated-receptors (PPARs) pathways. Moreover, it significantly increased the expression levels of TLR4, MyD88 and p-NF-κB p65 proteins and the contents of IL-1 and TNF-α, while the expression levels of occludin, claudin-1 and ZO-1 proteins decreased. These deleterious effects were significantly alleviated by MV and MRWD infusion. Our findings indicate that MRWD infusion helps alleviate diarrhoea, possibly by maintaining electrolyte homeostasis, improving the intestinal barrier integrity and inhibiting the TLR4/NF-κB axis.

中文翻译：

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加味人参乌梅汤减轻腹泻大鼠肠道屏障破坏[J].

著名中药人参乌梅汤加味被广泛用于治疗顽固性腹泻。然而，MRWD 调节腹泻的机制仍然未知。因此，我们检查了 MRWD 对腹泻模型中肠屏障完整性的保护作用。总共将 48 只雄性大鼠随机分配到四个治疗组：空白组（CK 组）、模型组（MC 组）、Medilac-Vita 组（MV 组）和中药组（MRWD 组）。经过 10 天的实验后，对血清和结肠样本进行了评估。腹泻指数、病理检查结果以及 D-乳酸和二胺氧化酶 (DAO) 含量的变化表明，腹泻的诱发导致肠道损伤，MV 和 MRWD 输注可改善肠道损伤。代谢组学分析确定了血清中的几种代谢物。一些关键代谢物，如磷酸、牛磺酸、可的松、白三烯 B4 和骨化三醇，被发现通过 MRWD 输注显着升高。重要的是，这些差异与矿物质吸收和代谢以及过氧化物酶体增殖物激活受体 (PPAR) 通路相关。此外，它显着增加TLR4、MyD88和p-NF-κB p65蛋白的表达水平以及IL-1和TNF-α的含量，而occludin、claudin-1和ZO-1蛋白的表达水平降低。MV 和 MRWD 输注显着减轻了这些有害影响。我们的研究结果表明，MRWD 输注有助于缓解腹泻，可能是通过维持电解质稳态，