There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products have recently been used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. The aim of this research was to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria has been determined. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-O-galloyl-glucose. The actively purified compound has potent antibacterial action against MRSA and clinical isolates of multidrug-resistant pathogenic bacteria. The actively purified compound has an effective antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. The combination of the active compound and β-lactam antibiotic (e.g., oxacillin) demonstrated synergistic action against MRSA, with an FIC index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active compound isolated from the plant is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.

中文翻译：

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来自景天的活性化合物对耐甲氧西林金黄色葡萄球菌及其临床分离株的协同抗菌活性。

由致病菌引起的医院获得性感染的报道越来越多，尤其是耐甲氧西林金黄色葡萄球菌(MRSA)。许多植物产品最近被用作探索针对不同类型人类病原菌的抗菌剂的天然手段。本研究的目的是从景天中分离和鉴定一种活性分子可能对 MRSA 的各种临床分离株具有抗菌活性。已经确定了不同提取物组分对多种致病菌的最低抑制浓度 (MIC)。NMR 分析表明，经 HPLC 纯化的化合物的结构为 1,2,4,6-四-O-没食子酰-葡萄糖。活性纯化的化合物对 MRSA 和多重耐药致病菌的临床分离株具有有效的抗菌作用。活性纯化的化合物对多重耐药致病菌，即MRSA及其临床分离株具有有效的抗菌活性。活性化合物和 β-内酰胺类抗生素（例如苯唑西林）的组合显示出对 MRSA 的协同作用，FIC 指数为 0.281。目前的研究揭示了一种使用植物材料对抗由多重耐药细菌引起的发病机制的替代方法。此外，使用从植物中分离出的活性化合物与抗生素相结合的组合方法已被证明是协同消灭病原体的成功方法。