Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer and is responsible for 20‑50% of thyroid cancer‑associated deaths. The absence of response to conventional treatments makes the search for novel therapeutics a clinical challenge. In the present study, the effects of 15,16‑dihydrotanshinone I (DHT), a tanshinone extracted from Salvia miltiorrhiza Bunge (Danshen), which has previously been shown to possess anticancer activity, were examined in two human ATC cell lines. DHT significantly reduced cell viability, which was coupled with an increase in apoptosis. DHT administration also reduced the colony‑forming ability and proliferation of these cells in soft agar and downregulated the expression of epithelial‑to‑mesenchymal transition‑related genes. In addition, DHT significantly reduced MAD2 expression, a target of HuR with a relevant role in ATC. Finally, cotreatment with cisplatin and DHT has a greater effect on cell viability than each compound alone. In conclusion, to the best of our knowledge, the present study is the first to demonstrate that DHT exerts antitumor effects on ATC cells by reducing MAD2 expression levels. Moreover, a synergistic effect of DHT with cisplatin was shown. Further in vivo studies are required to assess this phytochemical compound as a potential adjuvant for the treatment of ATC.

中文翻译：

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二氢丹参酮发挥抗肿瘤作用，改善顺铂对间变性甲状腺癌细胞的作用。

甲状腺未分化癌 (ATC) 是最具侵袭性的甲状腺癌类型，占甲状腺癌相关死亡的 20-50%。对常规治疗没有反应使得寻找新的治疗方法成为一项临床挑战。在本研究中，从丹参中提取的丹参酮 15,16-二氢丹参酮 I (DHT) 的作用Bunge (Danshen) 先前已被证明具有抗癌活性，在两种人 ATC 细胞系中进行了检查。DHT 显着降低细胞活力，同时增加细胞凋亡。DHT 给药还降低了这些细胞在软琼脂中的集落形成能力和增殖能力，并下调了上皮间质转化相关基因的表达。此外，DHT 显着降低MAD2表达，这是 HuR 的一个靶点，在 ATC 中具有相关作用。最后，与单独使用每种化合物相比，使用顺铂和 DHT 共同处理对细胞活力的影响更大。总之，据我们所知，本研究首次证明 DHT 通过降低MAD2对 ATC 细胞发挥抗肿瘤作用。表达水平。此外，显示了 DHT 与顺铂的协同作用。需要进一步的体内研究来评估这种植物化学化合物作为治疗 ATC 的潜在佐剂。