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Modulation of fatty acid elongation in cockroaches sustains sexually dimorphic hydrocarbons and female attractiveness.
PLOS Biology ( IF 7.8 ) Pub Date : 2021-07-27 , DOI: 10.1371/journal.pbio.3001330
Xiao-Jin Pei 1 , Yong-Liang Fan 1 , Yu Bai 2 , Tian-Tian Bai 1 , Coby Schal 3 , Zhan-Feng Zhang 1 , Nan Chen 2 , Sheng Li 2 , Tong-Xian Liu 1
Affiliation  

Insect cuticular hydrocarbons (CHCs) serve as important intersexual signaling chemicals and generally show variation between the sexes, but little is known about the generation of sexually dimorphic hydrocarbons (SDHCs) in insects. In this study, we report the molecular mechanism and biological significance that underlie the generation of SDHC in the German cockroach Blattella germanica. Sexually mature females possess more C29 CHCs, especially the contact sex pheromone precursor 3,11-DimeC29. RNA interference (RNAi) screen against the fatty acid elongase family members combined with heterologous expression of the genes in yeast revealed that both BgElo12 and BgElo24 were involved in hydrocarbon (HC) production, but BgElo24 is of wide catalytic activities and is able to provide substrates for BgElo12, and only the female-enriched BgElo12 is responsible for sustaining female-specific HC profile. Repressing BgElo12 masculinized the female CHC profile, decreased contact sex pheromone level, and consequently reduced the sexual attractiveness of female cockroaches. Moreover, the asymmetric expression of BgElo12 between the sexes is modulated by sex differentiation cascade. Specifically, male-specific BgDsx represses the transcription of BgElo12 in males, while BgTra is able to remove this effect in females. Our study reveals a novel molecular mechanism responsible for the formation of SDHCs and also provide evidences on shaping of the SDHCs by sexual selection, as females use them to generate high levels of contact sex pheromone.

中文翻译:

蟑螂中脂肪酸伸长的调节维持两性碳氢化合物和女性的吸引力。

昆虫表皮碳氢化合物 (CHC) 作为重要的两性间信号化学物质,通常表现出性别差异,但对昆虫中性二态碳氢化合物 (SDHC) 的产生知之甚少。在这项研究中,我们报告了德国蟑螂 Blattella Germanica 产生 SDHC 的分子机制和生物学意义。性成熟的女性拥有更多的 C29 CHC,尤其是接触性信息素前体 3,11-DimeC29。针对脂肪酸延长酶家族成员的 RNA 干扰 (RNAi) 筛选结合酵母中基因的异源表达表明 BgElo12 和 BgElo24 都参与了碳氢化合物 (HC) 的生产,但 BgElo24 具有广泛的催化活性并能够提供底物对于 BgElo12,并且只有富含女性的 BgElo12 负责维持女性特有的 HC 特征。抑制 BgElo12 会使雌性 CHC 轮廓变得男性化,降低接触性信息素水平,从而降低雌性蟑螂的性吸引力。此外,性别间 BgElo12 的不对称表达受性别分化级联调节。具体来说,雄性特异性 BgDsx 抑制雄性 BgElo12 的转录,而 BgTra 能够消除雌性中的这种影响。我们的研究揭示了一种负责 SDHC 形成的新分子机制,并为通过性选择塑造 SDHC 提供了证据,因为女性使用它们来产生高水平的接触性信息素。降低接触性信息素水平,从而降低雌性蟑螂的性吸引力。此外,性别间 BgElo12 的不对称表达受性别分化级联调节。具体来说,雄性特异性 BgDsx 抑制雄性 BgElo12 的转录,而 BgTra 能够消除雌性中的这种影响。我们的研究揭示了一种负责 SDHC 形成的新分子机制,并为通过性选择塑造 SDHC 提供了证据,因为女性使用它们来产生高水平的接触性信息素。降低接触性信息素水平,从而降低雌性蟑螂的性吸引力。此外,性别间 BgElo12 的不对称表达受性别分化级联调节。具体来说,雄性特异性 BgDsx 抑制雄性 BgElo12 的转录,而 BgTra 能够消除雌性中的这种影响。我们的研究揭示了一种负责 SDHC 形成的新分子机制,并为通过性选择塑造 SDHC 提供了证据,因为女性使用它们来产生高水平的接触性信息素。雄性特异性 BgDsx 抑制雄性 BgElo12 的转录,而 BgTra 能够消除雌性中的这种影响。我们的研究揭示了一种负责 SDHC 形成的新分子机制,并为通过性选择塑造 SDHC 提供了证据,因为女性使用它们来产生高水平的接触性信息素。雄性特异性 BgDsx 抑制雄性 BgElo12 的转录,而 BgTra 能够消除雌性中的这种影响。我们的研究揭示了一种负责 SDHC 形成的新分子机制,并为通过性选择塑造 SDHC 提供了证据,因为女性使用它们来产生高水平的接触性信息素。
更新日期:2021-07-27
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