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Genetic interrelationships of elite sorghum (Sorghum bicolor (L.) Moench) genotypes bred for Striga resistance and compatibility with Fusarium oxysporum f. sp. strigae (FOS) revealed by microsatellites
Acta Agriculturae Scandinavica Section B, Soil and Plant Science ( IF 1.7 ) Pub Date : 2021-07-27 , DOI: 10.1080/09064710.2021.1957142
Athenkosi Makebe 1 , Hussein Shimelis 1 , Admire I. T. Shayanowako 1
Affiliation  

ABSTRACT

Genetic diversity is key for breeding population development, hybrid breeding, heterotic grouping and genetic analysis, among others. The objective of this study was to examine genetic interrelationships among elite sorghum genotypes bred for resistance to Striga hermonthica Del. Benth and S. asiatica (L.) Kuntze and compatible with a biological control agent Fusarium oxysporum f. sp. strigae (FOS) against Striga using 22 polymorphic simple sequence repeat (SSR) or microsatellite markers. The number of alleles per locus detected by the markers varied from 2 to 7 with a mean value of 4.23. The polymorphic information content (PIC) of the SSR markers ranged from 0.15 to 0.84 with a mean of 0.57 showing moderate discriminatory power of the primers. The neighbour-joining clustering algorithm classified the genotypes into three main groups, revealing narrow to moderate variations existing among the test genotypes. Overall, the genetic diversity revealed by the study will be useful for designed crosses and pyramiding of broad-based resistance genes to control Striga for sustainable sorghum production.



中文翻译:

为独脚金抗性和与尖孢镰刀菌的相容性而培育的优良高粱 (Sorghum bicolor (L.) Moench) 基因型的遗传相互关系。sp. 由微卫星揭示的条纹 (FOS)

摘要

遗传多样性是育种种群发展、杂交育种、杂种优势分组和遗传分析等的关键。本研究的目的是检查高粱优良基因型之间的遗传相互关系,这些高粱基因型是为了抗性Striga hermonthica Del. BenthS. asiatica (L.) Kuntze 并与生物控制剂尖孢镰刀菌相容sp. 独脚( FOS ) 对抗独脚金使用 22 个多态性简单序列重复 (SSR) 或微卫星标记。标记检测到的每个基因座的等位基因数从 2 到 7 不等,平均值为 4.23。SSR 标记的多态信息含量 (PIC) 范围为 0.15 至 0.84,平均值为 0.57,表明引物的判别力中等。邻接聚类算法将基因型分为三个主要组,揭示了测试基因型之间存在的窄到中等变异。总体而言,该研究揭示的遗传多样性将有助于设计杂交和基础广泛的抗性基因的金字塔化,以控制独脚金以实现可持续的高粱生产。

更新日期:2021-07-28
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