Vincristine (Vin) is a well-known antitumor agent that frequently evokes neuropathic pain and decreases the quality of life of patients. Polysaccharides (GBP) extracted from Gastrodia elata Blume have been demonstrated to possess anti-inflammatory and neuroprotective effects in vivo; however, the effects of GBP on Vin-induced neuropathic pain remain unknown. The present study is aimed at exploring the alleviative potential of GBP against chemotherapy-evoked peripheral neuropathy to better understand and extend its pharmacological application. Vin was administered intraperitoneally to evoke neuropathic pain. GBP was orally administered for 21 days. The mechanical allodynia and thermal hyperalgesia were assessed using the Von Frey test and hot-plate test. Histopathological changes were assessed by hematoxylin and eosin staining. ELISA kits were used to measure the levels of inflammatory cytokines in the sciatic nerve, spinal cord, and dorsal root ganglion (DRG). qRT-PCR was employed to examine the expression of inflammatory cytokines and Sirtuin1 (SIRT1) in the sciatic nerve, spinal cord, and DRG. Our findings revealed that GBP treatment enhanced the paw withdrawal latency and paw withdrawal threshold and restored Vin-induced sciatic nerve damage in rats. GBP also attenuated the Vin-induced increase of proinflammatory cytokine levels, including IL-6, IL-8, TNF-α, IL-1β, and NF-κB. On the molecular level, treatment with GBP downregulated the mRNA levels of IL-6, IL-8, TNF-α, and IL-1β in the sciatic nerve, spinal cord, and DRG. Meanwhile, GBP increased SIRT1 activity and mRNA expression levels. Our data indicated that GBP exerted a potential protective effect against chemotherapy-induced neuropathic pain which might be mediated via the inhibition of neuroinflammation.

中文翻译：

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天麻多糖通过抑制神经炎症减轻长春新碱诱发的神经性疼痛

长春新碱 (Vin) 是一种众所周知的抗肿瘤剂，它经常引起神经性疼痛并降低患者的生活质量。从天麻中提取的多糖 (GBP)已被证明在体内具有抗炎和神经保护作用; 然而，GBP 对 Vin 诱导的神经性疼痛的影响仍然未知。本研究旨在探索 GBP 对化疗诱发的周围神经病变的缓解潜力，以更好地了解和扩展其药理应用。腹腔内给予Vin以引起神经性疼痛。GBP 口服给药 21 天。使用 Von Frey 试验和热板试验评估机械异常性疼痛和热痛觉过敏。通过苏木精和伊红染色评估组织病理学变化。ELISA 试剂盒用于测量坐骨神经、脊髓和背根神经节 (DRG) 中炎性细胞因子的水平。qRT-PCR 用于检查坐骨神经、脊髓和 DRG 中炎性细胞因子和 Sirtuin1 (SIRT1) 的表达。我们的研究结果表明，GBP 治疗可提高大鼠缩爪潜伏期和缩爪阈值，并恢复 Vin 诱导的大鼠坐骨神经损伤。GBP 还减弱了 Vin 诱导的促炎细胞因子水平的增加，包括 IL-6、IL-8、TNF-α、 IL-1 β和 NF- κ B. 在分子水平上，GBP 治疗可下调坐骨神经、脊髓中 IL-6、IL-8、TNF- α和 IL-1 β的 mRNA 水平和DRG。同时，GBP 增加了 SIRT1 活性和 mRNA 表达水平。我们的数据表明，GBP 对化疗引起的神经性疼痛具有潜在的保护作用，这可能是通过抑制神经炎症来介导的。