This study is based on UHPLC-Q-TOF/MS and fragment ions to achieve classification and identification of alkaloids and flavonoids in Sophora flavescens. By reviewing the available and relevant literature, the mass fragmentation rules of alkaloids and flavonoids were summarized. 0.1% formic acid water (A) and acetonitrile (B) were used as mobile phases. 37 chemical constituents were identified, including 13 alkaloids and 24 flavonoids. This research method offers a complete strategy based on the fragmentation information of characteristic fragment ions and neutral loss obtained by MS/MS to characterize the chemical composition of Sophora flavescens.

中文翻译：

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UHPLC-Q-TOF/MS 测定苦参中的生物碱和黄酮类化合物

本研究基于UHPLC-Q-TOF/MS和碎片离子实现苦参中生物碱和黄酮类化合物的分类鉴定。通过查阅现有及相关文献，总结了生物碱和黄酮类物质的质量破碎规律。0.1% 甲酸水 (A) 和乙腈 (B) 用作流动相。共鉴定出37种化学成分，其中生物碱13种，黄酮类24种。该研究方法提供了基于MS/MS获得的特征碎片离子碎裂信息和中性丢失来表征苦参化学成分的完整策略。