Waiting lists that continue to grow and the lack of organs available for transplantation necessitate the use of marginal livers, such as fatty livers. Since steatotic livers are more susceptible to damage from ischemia and reperfusion, it was investigated whether fatty livers with different lipidomic profiles show a different outcome when subjected to long-term cold storage preservation. Eight-week-old male Wistar rats fed for 2 weeks by a methionine-choline-deficient (MCD) diet or control diet were employed in this study. Livers were preserved in a University of Wisconsin (UW) solution at 4 °C for 6, 12 or 24 h and, after washout, reperfused for 2 h with a Krebs-Henseleit buffer at 37 °C. Hepatic enzyme release, bile production, O2-uptake, and portal venous pressure (PVP) were evaluated. The liver fatty acid profile was evaluated by a gas chromatography-mass spectrometry (GC/MS). MCD rats showed higher LDH and AST levels with respect to the control group. When comparing MCD livers preserved for 6, 12 or 24 h, no differences in enzyme release were found during both the washout or the reperfusion period. The same trend occurred for O2-uptake, PVP, and bile flow. A general decrease in SFA and MUFA, except for oleic acid, and a decrease in PUFA, except for arachidonic, eicosadienoic, and docosahexanaeoic acids, were found in MCD rats when compared with control rats. Moreover, the ratio between SFA and the various types of unsaturated fatty acids (UFA) was significantly lower in MCD rats. Although prolonged cold ischemia negatively affects the graft outcome, our data suggest that the quality of lipid constituents could influence liver injury during cold storage: the lack of an increased hepatic injury in MCD may be justified by low SFA, which likely reduces the deleterious tendency toward lipid crystallization occurring under cold ischemia.

中文翻译：

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长期冷藏保存对缺乏蛋氨酸和胆碱饮食的大鼠的脂肪肝没有影响

等待名单不断增加，并且缺乏可用于移植的器官，因此需要使用边缘肝脏，例如脂肪肝。由于脂肪肝更容易受到缺血和再灌注的损害，因此研究了具有不同脂质组学特征的脂肪肝在长期冷藏保存时是否表现出不同的结果。本研究采用八周大的雄性 Wistar 大鼠，用蛋氨酸胆碱缺乏 (MCD) 饮食或对照饮食喂养 2 周。将肝脏在 4°C 的威斯康星大学 (UW) 溶液中保存 6、12 或 24 小时，冲洗后，用 Krebs-Henseleit 缓冲液在 37°C 下再灌注 2 小时。评估肝酶释放、胆汁产生、氧气摄取和门静脉压（PVP）。通过气相色谱-质谱法（GC/MS）评估肝脏脂肪酸谱。与对照组相比，MCD 大鼠表现出更高的 LDH 和 AST 水平。当比较保存 6、12 或 24 小时的 MCD 肝脏时，在冲洗或再灌注期间未发现酶释放的差异。O2 吸收、PVP 和胆汁流量也出现相同的趋势。与对照大鼠相比，MCD 大鼠的 SFA 和 MUFA（油酸除外）普遍降低，PUFA（花生四烯酸、二十碳二烯酸和二十二碳六烯酸除外）普遍降低。此外，MCD 大鼠中 SFA 与各类不饱和脂肪酸 (UFA) 的比率显着降低。尽管长时间的冷缺血会对移植结果产生负面影响，但我们的数据表明，脂质成分的质量可能会影响冷藏期间的肝损伤：MCD 中肝损伤的增加可能是由于低 SFA 所致，这可能会减少对肝脏的有害倾向。冷缺血下发生脂质结晶。