Polymerase chain reaction (PCR) is commonly used to amplify and quantify the nucleic acid segments typically using a benchtop thermocycler. To automate, integrate, and miniaturize the PCR process, several strategies have been studied in a microfluidic environment. Among them, continuous-flow-based microfluidic PCR allows fast thermal cycling using minuscule volume, in a minimal reaction time with multiplexing. The objective was to develop a microfluidic device with a soft-lithographically fabricated continuous-flow serpentine microchannel for DNA amplification executed on a separately designed portable, easy-to-use, low-cost, automated, and miniaturized temperature controller platform (TCP). Direct laser writer (DLW) was used for developing a master on glass using a dry-film photoresist (DFR). Further, a PDMS-based microfluidic device, with dimensions 30 (L) × 0.32 mm² (W) × 35 µm (H), was developed which was bonded on glass using oxygen plasma. The portable device exhibits key features of live data streaming using an IoT platform enabling easy data accessing, monitoring and storage onto the smartphone. The temperature sensitivity of the device was ± 0.5 °C and the maximum achievable temperature was 300 °C. The microfluidic device was placed on TCP. A 20 µL of reaction volume was introduced using an automated syringe pump at various flow rates. As a proof-of-concept, the rat GAPDH gene of the 594-base pair was successfully amplified on the proposed platform which was validated using the gel electrophoresis method. Finally, the results obtained from the proposed device were compared with the conventional thermocycler which showed promising performance and novelty exists in the significant reduction of required amplification time with good device efficiency and low-power consumption.

聚合酶链反应 (PCR) 通常用于扩增和量化核酸片段，通常使用台式热循环仪。为了自动化、集成和小型化 PCR 过程，已经在微流体环境中研究了几种策略。其中，基于连续流的微流体 PCR 允许使用极小的体积进行快速热循环，在最短的反应时间内进行多路复用。目标是开发一种具有软光刻制造的连续流动蛇形微通道的微流体装置，用于在单独设计的便携式、易于使用、低成本、自动化和小型化的温度控制器平台 (TCP) 上执行 DNA 扩增。直接激光刻录机 (DLW) 用于使用干膜光刻胶 (DFR) 在玻璃上显影母版。此外，基于 PDMS 的微流体装置，^{开发了 2} (W) × 35 µm (H)，使用氧等离子体将其粘合在玻璃上。该便携式设备展示了使用 IoT 平台进行实时数据流传输的主要功能，可轻松将数据访问、监控和存储到智能手机上。该器件的温度灵敏度为 ± 0.5 °C，可达到的最高温度为 300 °C。微流体装置被放置在 TCP 上。使用自动注射泵以不同流速引入 20 µL 反应体积。作为概念验证，大鼠GAPDH594 碱基对的基因在所提出的平台上成功扩增，该平台使用凝胶电泳方法进行了验证。最后，从所提出的设备获得的结果与传统的热循环仪进行了比较，传统的热循环仪在显着减少所需的放大时间、良好的设备效率和低功耗方面显示出有希望的性能和新颖性。