In the article “Flow cytometric evaluation of the neutrophil compartment in COVID-19 at hospital presentation: A normal response to an abnormal situation,” by Roy Spijkerman, Suzanne H. Bongers, Bas J. J. Bindels, et al., appearing in the Journal of Leukocyte Biology, January 2021, 109(1):99-114; doi: 10.1002/JLB.5COVA0820-520RRR, an error occurred in the gating strategy of Figure 1 that erroneously identifies eosinophils and myelocytes in different gates. The eosinopenia in the blood of the COVID19 patients, together with the relatively high number of myelocytes, leads to cross-contamination in the respective gates, as both cell types are CD16^low and CD11b ^bright to various degrees.

This error does not affect the neutrophil data, which are the main subject of the study. The authors have provided corrected figures that combine these two separate gates into one eosinophil/myelocyte gate, and do not allow discrimination of both cell types. Original figures 3E, 6C, and 6D should be ignored, as they are based on the cross-contamination in eosinophil and myelocyte gates in the original Figure 1. The correct figures are shown below.

The authors regret the error.

在 Roy Spijkerman、Suzanne H. Bongers、Bas JJ Bindel 等人发表在 Journal of白细胞生物学，2021年1月，109(1):99-114；doi: 10.1002/JLB.5COVA0820-520RRR，图 1 的门控策略发生错误，错误地识别了不同门控中的嗜酸性粒细胞和粒细胞。COVID19 患者血液中的嗜酸性粒细胞减少，加上相对较多的髓细胞，导致相应门中的交叉污染，因为两种细胞类型的 CD16^低，CD11b^亮不同程度。

这个错误不会影响中性粒细胞数据，这是研究的主要主题。作者提供了更正的数字，将这两个单独的门组合成一个嗜酸性粒细胞/骨髓细胞门，并且不允许区分两种细胞类型。原始图 3E、6C 和 6D 应被忽略，因为它们基于原始图 1 中嗜酸性粒细胞和髓细胞门的交叉污染。正确的数字如下所示。

作者对这个错误感到遗憾。