The wound bacterial infections have become an intractable problem to human health. Multifunctional wound dressing materials with excellent adhesion, antibacterial as well as wound healing properties are still desperately needed to resist wound infection. In this work, an injectable hydrogel has been prepared, which chitosan and hyaluronic acid were cross-linked by aldehyde modified polyethylene glycol (PEG). For improving the antibacterial property, S-nitrosothiols was used as nitric oxide (NO) donor to provide a sustained NO release. The gelation time, microstructure, pH responsiveness, injectability, self-healing property as well as NO release profile were characterized. Quick and efficient antibacterial potency towards E. coli and S. epidermidis was confirmed in vitro with contact-killing assays. The co-culturing experiment with NIH 3 T3, HFF-1 and bEnd.3 cells indicated excellent biocompatibility of the hydrogels. Most importantly, the hydrogels showed the ability to accelerate the cell migration, demonstrating a potential to promote wound healing by scratch-wound assay. The current work provided a new type of injectable self-healing hydrogel for antibacterial function and infectious skin repair, leading to great potentials for infected wound healing.

伤口细菌感染已成为困扰人类健康的难题。仍然迫切需要具有优异粘附性、抗菌性和伤口愈合性能的多功能伤口敷料材料来抵抗伤口感染。在这项工作中，制备了一种可注射水凝胶，其中壳聚糖和透明质酸通过醛改性聚乙二醇 (PEG) 交联。为了提高抗菌性能，S-亚硝基硫醇被用作一氧化氮 (NO) 供体以提供持续的 NO 释放。表征了凝胶时间、微观结构、pH 响应性、可注射性、自愈性能以及 NO 释放曲线。对大肠杆菌和表皮葡萄球菌具有快速有效的抗菌效力在体外通过接触致死试验得到证实。与 NIH 3 T3、HFF-1 和 bEnd.3 细胞的共培养实验表明水凝胶具有出色的生物相容性。最重要的是，水凝胶显示出加速细胞迁移的能力，证明了通过划伤试验促进伤口愈合的潜力。目前的工作提供了一种具有抗菌功能和感染性皮肤修复的新型可注射自愈合水凝胶，为感染伤口愈合带来了巨大潜力。