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Extensive alternative splicing triggered by mitonuclear mismatch in naturally introgressed Rhinolophus bats
Ecology and Evolution ( IF 2.3 ) Pub Date : 2021-07-28 , DOI: 10.1002/ece3.7966 Wenli Chen 1 , Xiuguang Mao 1, 2
Ecology and Evolution ( IF 2.3 ) Pub Date : 2021-07-28 , DOI: 10.1002/ece3.7966 Wenli Chen 1 , Xiuguang Mao 1, 2
Affiliation
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Mitochondrial function needs strong interactions of mitochondrial and nuclear (mitonuclear) genomes, which can be disrupted by mitonuclear mismatch due to mitochondrial DNA (mtDNA) introgression between two formerly isolated populations or taxa. This mitonuclear disruption may cause severe cellular stress in mismatched individuals. Gene expression changes and alternative splicing (AS) are two important transcriptional regulations to respond to environmental or cellular stresses. We previously identified a naturally introgressed population in the intermediate horseshoe bat (Rhinolophus affinis). Individuals from this population belong to R. a. himalayanus and share almost identical nuclear genetic background; however, some of them had mtDNA from another subspecies (R. a. macrurus). With this unique natural system, we examined gene expression changes in six tissues between five mitonuclear mismatched and five matched individuals. A small number of differentially expressed genes (DEGs) were identified, and functional enrichment analysis revealed that most DEGs were related to immune response although some may be involved in response to oxidative stress. In contrast, we identified extensive AS events and alternatively spliced genes (ASGs) between mismatched and matched individuals. Functional enrichment analysis revealed that multiple ASGs were directly or indirectly associated with energy production in mitochondria which is vital for survival of organism. To our knowledge, this is the first study to examine the role of AS in responding to cellular stress caused by mitonuclear mismatch in natural populations. Our results suggest that AS may play a more important role than gene expression regulation in responding to severe environmental or cellular stresses.
中文翻译：
自然渐渗菊头蝠中线粒体核错配引发的广泛选择性剪接
线粒体功能需要线粒体和核基因组之间的强烈相互作用，而线粒体 DNA (mtDNA) 渗入两个先前孤立的种群或类群之间，导致线粒体核不匹配，从而破坏线粒体功能。这种线粒体核破坏可能会对不匹配的个体造成严重的细胞应激。基因表达变化和选择性剪接（AS）是响应环境或细胞应激的两个重要转录调控。我们之前在中间马蹄蝠（ Rhinolophus affinis ）中发现了一个自然渗入的种群。该群体中的个体属于R. a 。喜马拉雅山和喜马拉雅山具有几乎相同的核遗传背景；然而，其中一些具有来自另一个亚种（ R.a.macrurus ）的线粒体DNA。通过这种独特的自然系统，我们检查了五个线粒体核不匹配和五个匹配个体之间的六个组织中的基因表达变化。鉴定出了少量差异表达基因（DEG），功能富集分析显示大多数 DEG 与免疫反应有关，尽管有些可能与氧化应激反应有关。相比之下，我们在不匹配和匹配个体之间发现了广泛的 AS 事件和选择性剪接基因 (ASG)。功能富集分析表明，多种 ASG 与线粒体的能量产生直接或间接相关，这对生物体的生存至关重要。据我们所知，这是第一项研究 AS 在应对自然群体中线粒体核不匹配引起的细胞应激中的作用的研究。 我们的结果表明，在应对严重的环境或细胞应激方面，AS 可能比基因表达调控发挥更重要的作用。
更新日期：2021-09-09
中文翻译：
自然渐渗菊头蝠中线粒体核错配引发的广泛选择性剪接
线粒体功能需要线粒体和核基因组之间的强烈相互作用，而线粒体 DNA (mtDNA) 渗入两个先前孤立的种群或类群之间，导致线粒体核不匹配，从而破坏线粒体功能。这种线粒体核破坏可能会对不匹配的个体造成严重的细胞应激。基因表达变化和选择性剪接（AS）是响应环境或细胞应激的两个重要转录调控。我们之前在中间马蹄蝠（ Rhinolophus affinis ）中发现了一个自然渗入的种群。该群体中的个体属于R. a 。喜马拉雅山和喜马拉雅山具有几乎相同的核遗传背景；然而，其中一些具有来自另一个亚种（ R.a.macrurus ）的线粒体DNA。通过这种独特的自然系统，我们检查了五个线粒体核不匹配和五个匹配个体之间的六个组织中的基因表达变化。鉴定出了少量差异表达基因（DEG），功能富集分析显示大多数 DEG 与免疫反应有关，尽管有些可能与氧化应激反应有关。相比之下，我们在不匹配和匹配个体之间发现了广泛的 AS 事件和选择性剪接基因 (ASG)。功能富集分析表明，多种 ASG 与线粒体的能量产生直接或间接相关，这对生物体的生存至关重要。据我们所知，这是第一项研究 AS 在应对自然群体中线粒体核不匹配引起的细胞应激中的作用的研究。 我们的结果表明，在应对严重的环境或细胞应激方面，AS 可能比基因表达调控发挥更重要的作用。
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