Lysophosphatidic acid (LPA) is a bioactive lysophospholipid that causes neuronal growth cones to collapse and neurites to retract through a RhoA-ROCK mediated pathway. It has been reported that the NSAID ibuprofen improves regeneration after spinal cord injury through a mechanism of inhibiting RhoA. This leads to the hypothesis that ibuprofen should block LPA-mediated growth cone collapse. We tested this hypothesis by treating embryonic chick retinal neurons with ibuprofen followed by LPA. Retinal growth cones collapsed with LPA in the presence of ibuprofen similar to control; however, growth cone collapse was effectively blocked by a ROCK inhibitor. Thus, our results do not support the designation of ibuprofen as a direct RhoA inhibitor.

溶血磷脂酸 (LPA) 是一种生物活性溶血磷脂，可通过 RhoA-ROCK 介导的途径导致神经元生长锥塌陷和神经突收缩。据报道，NSAID 布洛芬通过抑制 RhoA 的机制改善脊髓损伤后的再生。这导致假设布洛芬应该阻止 LPA 介导的生长锥塌陷。我们通过先用布洛芬和 LPA 处理雏鸡视网膜神经元来检验这一假设。与对照组相似，在布洛芬存在的情况下，LPA 使视网膜生长锥塌陷；然而，ROCK 抑制剂有效阻止了生长锥塌陷。因此，我们的结果不支持将布洛芬指定为直接 RhoA 抑制剂。