Abstract

In this work, we prepared silver nanoparticles (AgNPs)-loaded poly(2,5-dimethoxyaniline;PDMA) nanocomposite via a simple chemical oxidative method to develop new effective biocidal and anticancer agents systems. In situ UV–vis and fluorescence spectroscopy has been used to monitor the formation of PDMA/Ag is strongly dependent on the concentration of PDMA in the present system. The FTIR and XRD studies exhibit that the rate of polymer formation and oxidation state of PDMA/Ag nanocomposite, which confirmed the AgNPs is strongly binding with the PDMA matrix. SEM and EDAX analysis revealed the presence of uniform size and the highest percentage of AgNPs (42.1%) in the PDMA/Ag nanocomposite. TEM and size distribution analysis revealed the presence of spherical AgNPs with an average diameter of 25 nm in the PDMA/Ag nanocomposite. In addition, the PDMA polymer with embedded AgNPs exhibited excellent antibacterial performance against Escherichia coli and Staphylococcus aureus. Interestingly, the prepared PDMA/Ag nanocomposite exhibited enhanced bactericidal performance compared with the PDMA polymer, presumably because of the antibacterial properties of the AgNPs substrate. The anticancer effects of combined treatment with PDMA polymer and AgNPs were evaluated using a series of cellular and biochemical assays. The findings from this study revealed that the PDMA/Ag nanocomposite treatment significantly inhibited cell viability and proliferation of the human cervical cancer cell line (HeLa). The PDMA/Ag nanocomposite exhibits better antibacterial and anticancer activities compare to pristine PDMA.

摘要

在这项工作中，我们通过简单的化学氧化方法制备了负载银纳米粒子 (AgNPs) 的聚（2,5-二甲氧基苯胺；PDMA）纳米复合材料，以开发新的有效杀生物和抗癌剂系统。原位 UV-vis 和荧光光谱已用于监测 PDMA/Ag 的形成，这在很大程度上取决于本系统中 PDMA 的浓度。FTIR 和 XRD 研究表明 PDMA/Ag 纳米复合材料的聚合物形成速率和氧化态，这证实了 AgNPs 与 PDMA 基质强烈结合。SEM 和 EDAX 分析显示 PDMA/Ag 纳米复合材料中存在均匀尺寸和最高百分比的 AgNPs (42.1%)。TEM 和尺寸分布分析表明，PDMA/Ag 纳米复合材料中存在平均直径为 25 nm 的球形 AgNP。此外，大肠杆菌和金黄色葡萄球菌。有趣的是，与 PDMA 聚合物相比，制备的 PDMA/Ag 纳米复合材料表现出增强的杀菌性能，这可能是因为 AgNPs 基材的抗菌性能。使用一系列细胞和生化分析评估了 PDMA 聚合物和 AgNPs 联合治疗的抗癌效果。这项研究的结果表明，PDMA/Ag 纳米复合材料处理显着抑制了人宫颈癌细胞系 (HeLa) 的细胞活力和增殖。与原始 PDMA 相比，PDMA/Ag 纳米复合材料表现出更好的抗菌和抗癌活性。