Protein inhibitor of activated signal transducer and activator of transcription (PIAS) family protein involved in gene transcriptional regulation acts as negative regulator in Janus kinase-signal transducer and activator of transcription (JAK/STAT) signaling pathway. But until now, the roles of PIAS in fish are not clear. In this study, we identified the two mammalian PIAS1 orthologs from Ctenopharyngodon idellus, namely CiPIAS1a and CiPIAS1b, respectively. They can respond to the stimulation from Polyribocytidylic acid (Poly I:C), Grass Carp Reovirus (GCRV) and Lipopolysaccharides (LPS) respectively, so we suggested that they could participate in interferon (IFN)-mediated antiviral and antibacterial immune response. The subcellular localization and nuclear cytoplasm extraction showed that CiPIAS1a and CiPIAS1b were mainly distributed in the nucleus. In addition, Co-IP showed that they separately inhibited the phosphorylation of STAT1 via interacting with it, which leads to the reduction of IFN1 expression.

参与基因转录调控的激活信号转导和转录激活因子 (PIAS) 家族蛋白的蛋白抑制剂在 Janus 激酶信号转导和转录激活因子 (JAK/STAT) 信号通路中充当负调节因子。但直到现在，PIAS 在鱼类中的作用尚不清楚。在这项研究中，我们确定了来自Ctenopharyngodon idellus的两个哺乳动物 PIAS1 直系同源物，即CiPIAS1a和CiPIAS1b， 分别。它们可以分别对多核糖胞苷酸（Poly I:C）、草鱼呼肠孤病毒（GCRV）和脂多糖（LPS）的刺激产生反应，因此我们认为它们可以参与干扰素（IFN）介导的抗病毒和抗菌免疫反应。亚细胞定位和核质提取表明CiPIAS1a和CiPIAS1b主要分布在细胞核中。此外，Co-IP 表明它们通过与 STAT1 相互作用分别抑制了 STAT1 的磷酸化，从而导致 IFN1 表达降低。