In this study, iron oxide nanoparticles (NPs) had been prepared by co-precipitation method. In order to reduce their toxicity and increase stability, prepared iron oxide was coated with gum Arabic. Gum Arabic is preferred over synthetic materials due to their non-toxicity, low cost and availability. Characterization of coated and non-coated iron oxide NPs had been performed by spectrophotometer, Fourier transfer infra-red spectrophotometer (FTIR), Zeta potential, X-ray diffraction (XRD) and field emission scanning electron microscopic (FE-SEM). The fabricated nanoparticles appeared purity and crystalline nature by XRD, with diameter average of 27.01 nm and 55.12 nm for iron oxide NPs and iron oxide NPs coated with gum Arabic, respectively. On the other hand, four biological activities of coated and non-coated iron oxide had been investigated. High removal of methylene blue pollutant dye (46%) was observed with iron oxide NPs, while removal percentage was 22.6 performed by iron oxide NPs coated with gum Arabic within 72 h. Iron oxide NPs revealed high inhibition zones of 27.5 nm and 30 mm, at 1000 μg/ml, against S. aureus and E. coli, respectively, while coated iron oxide NPs with gum Arabic revealed low antibacterial activity against both examined bacteria even when used at 1000 μg/ml. The hemolytic activity of prepared NPs had been determined. The hemolytic percentage was increased whenever concentrations of nanoparticles increased. Lower hemolytic percentages were 69.76 and 50.98 for iron oxide NPs and iron oxide NPs coated with gum Arabic were observed at a concentration of 250 μg/ml. Finally, cytotoxic activity was estimated against MCF-7 cell line and normal cell line WRL68 by MTT assay. A decrease in MCF-7 viability to 65.1% was observed when 400 μg/ml of iron oxide NPs was used, while WRL68 viability was 75.03%. Iron oxide NPs coated with gum Arabic revealed significant reduction in MCF-7 and WRL68 viability to 69.90% and 80.05%, respectively, when 400 μg/ml of nanoparticles was applied.

中文翻译：

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阿拉伯树胶涂层氧化亚铁纳米粒子的生物活性

在这项研究中，通过共沉淀法制备了氧化铁纳米颗粒（NPs）。为了降低它们的毒性和增加稳定性，制备的氧化铁涂有阿拉伯树胶。阿拉伯树胶因其无毒、低成本和可用性而优于合成材料。已通过分光光度计、傅里叶转移红外分光光度计 (FTIR)、Zeta 电位、X 射线衍射 (XRD) 和场发射扫描电子显微镜 (FE-SEM) 对涂层和未涂层​​氧化铁纳米颗粒进行了表征。制备的纳米粒子通过 XRD 显示纯度和结晶性质，氧化铁纳米粒子和涂有阿拉伯树胶的氧化铁纳米粒子的平均直径分别为 27.01 nm 和 55.12 nm。另一方面，研究了涂层和未涂层​​氧化铁的四种生物活性。氧化铁纳米颗粒对亚甲基蓝污染物染料的去除率很高（46%），而涂有阿拉伯树胶的氧化铁纳米颗粒在 72 小时内去除率达到 22.6。氧化铁纳米颗粒在 1000 时显示出 27.5 nm 和 30 mm 的高抑制区μ克/毫升，反对金黄色葡萄球菌和大肠杆菌，分别，而用阿拉伯树胶包裹的氧化铁纳米颗粒即使在 1000μ克/毫升。已确定制备的 NPs 的溶血活性。每当纳米颗粒浓度增加时，溶血百分比增加。氧化铁纳米颗粒的溶血百分比较低，分别为 69.76 和 50.98，并且在浓度为 250 时观察到涂有阿拉伯树胶的氧化铁纳米颗粒μ克/毫升。最后，通过 MTT 测定估计对 MCF-7 细胞系和正常细胞系 WRL68 的细胞毒活性。当 400μ使用 g/ml 的氧化铁 NPs，而 WRL68 的存活率为 75.03%。涂有阿拉伯树胶的氧化铁纳米颗粒显示，当 400 时，MCF-7 和 WRL68 的活力分别显着降低至 69.90% 和 80.05%μ施加了g/ml的纳米颗粒。