Abstract

The best known polymers (alginate, carrageenan, and acrylamide) were examined for their potential use as carriers of mycelium cells of Aspergillus ochraceus VKM-F4104D, a producer of fibrinolytic protease activator of protein C in human blood plasma during its deep cultivation. The morpho-cytological features of the development of A. ochraceus VKM-F4104D mycelium in granules of carrageenan and calcium alginate are shown. The conditions for the cultivation of micromycete immobilized in granules of 3.5% calcium alginate were optimized. The immobilized cells of the producer mycelium demonstrated increased stability and were able to synthesize protein-C protease-activator in blood plasma at the maximum level for 10 or more days. In addition, immobilized mycelial cells during cultivation can be used repeatedly (up to five cycles).

中文翻译：

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微菌赭曲霉 VKM-F4104D 固定在聚合物载体中对血浆蛋白 C 纤溶蛋白酶激活剂产生的影响

摘要

研究了最著名的聚合物（藻酸盐、角叉菜胶和丙烯酰胺）作为赭曲霉VKM-F4104D菌丝体细胞载体的潜在用途，曲霉属 VKM-F4104D 是一种在深度培养过程中人血浆中蛋白 C 的纤溶蛋白酶激活剂的生产者。A. ochraceus发育的形态细胞学特征显示了角叉菜胶和海藻酸钙颗粒中的 VKM-F4104D 菌丝体。优化了3.5%海藻酸钙颗粒固定化微菌的培养条件。生产菌丝体的固定化细胞表现出更高的稳定性，并且能够在血浆中以最高水平合成蛋白-C 蛋白酶激活剂 10 天或更长时间。此外，培养过程中固定的菌丝细胞可以重复使用（最多五个循环）。