Abstract

Dinitrosyl iron complexes with glutathione ligands (GS-DNICs) are a physiological form of nitric oxide that is stored and transported in the organism. In addition, these complexes can act as antioxidants and antiradical agents. It was shown that GS-DNICs protect hemoglobin (Hb) from oxidative modification by peroxynitrite. They prevent the formation of carbonyl Hb derivatives, the oxidation of tryptophan and tyrosine residues, degradation of the heme group, and the formation of interprotein cross-links. These complexes also inhibit the oxidation of dihydrorhodamine by peroxynitrite, which forms via the decomposition of 3-morpholinosyndonimine. In some cases, the antioxidant effect of GS-DNICs is comparable to that of reduced glutathione. The results allow GS-DNICs to be considered peroxynitrite interceptors and Hb protectors.

中文翻译：

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二亚硝基铁配合物与谷胱甘肽配体拦截过氧亚硝酸盐并保护血红蛋白免受氧化修饰

摘要

具有谷胱甘肽配体的二亚硝基铁络合物 (GS-DNIC) 是一氧化氮的生理形式，可在生物体中储存和运输。此外，这些复合物可以作为抗氧化剂和抗自由基剂。结果表明，GS-DNIC 可保护血红蛋白 (Hb) 免受过氧亚硝酸盐的氧化修饰。它们阻止羰基 Hb 衍生物的形成、色氨酸和酪氨酸残基的氧化、血红素基团的降解以及蛋白间交联的形成。这些复合物还抑制过氧亚硝酸盐对二氢罗丹明的氧化，过氧亚硝酸盐通过 3-吗啉代联亚胺的分解形成。在某些情况下，GS-DNIC 的抗氧化作用与还原型谷胱甘肽的抗氧化作用相当。结果允许 GS-DNIC 被视为过氧亚硝酸盐拦截器和 Hb 保护器。