Our aim was to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9. 150 human fecal samples were collected and used for isolation of vitamin producers-probiotics. 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes. As a result, three isolates were selected and their production of the B2 and B9 were confirmed by HPLC. The three isolates were identified on species level by sequencing their 16S rRNA gene which showed 100% identical to strains of Pediococcus acidilactici. Thus, they were named as P. acidilactici WNYM01, P. acidilactici WNYM02, P. acidilactici WNYM03 and submitted to the Genbank database with accession numbers. They met the probiotic criteria by expressing 90-95% survival rate at pH (2.0-9.0) and bile salt up to 2% for 3 h in addition to their antimicrobial activity against gram positive and negative microorganisms. They also showed no hemolytic activity and common pattern for antibiotic susceptibility. Our three strains were tested individually or in mixture in vivo on rat colitis model compared to ulcerative group. The strains were administrated orally to rats in daily dose containing CFU 109 for 14 days then followed by induction of colitis using acetic acid then the oral administration was continued for more four days. The histology results, the anti-inflammatory and anti-oxidative stress biomarkers showed the protective role of the strains compared to the ulcerative group. As a conclusion, we introduce novel three probiotic candidates for pharmaceutical preparations and health applications.

中文翻译：

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通过新的乳酸片球菌菌株抑制大鼠醋酸诱导的结肠炎，这种菌株是从人类肠道微生物群中恢复的维生素生产者。

我们的目标是分离、鉴定和表征作为维生素生产者的益生菌，特别是 B2 和 B9。收集了 150 份人类粪便样本，用于分离维生素生产者 - 益生菌。选择 49 个分离株用于通过 PCR 筛选其基因组中是否存在核黄素和叶酸基因。结果，选择了三个分离株，并通过 HPLC 确认了它们产生的 B2 和 B9。通过对它们的 16S rRNA 基因进行测序，在物种水平上鉴定了这三个分离株，该基因显示与乳酸片球菌菌株 100% 相同。因此，它们被命名为乳酸假单胞菌 WNYM01、乳酸假单胞菌 WNYM02、乳酸假单胞菌 WNYM03，并以登录号提交到 Genbank 数据库。它们通过在 pH 值 (2.0-9. 0) 和胆汁盐最多 2% 持续 3 小时，此外它们对革兰氏阳性和阴性微生物具有抗菌活性。它们也没有显示出溶血活性和抗生素敏感性的常见模式。与溃疡组相比，我们的三种菌株在大鼠结肠炎模型上单独或混合进行了体内测试。将菌株以含有 CFU 109 的日剂量口服给药至大鼠 14 天，然后使用乙酸诱导结肠炎，然后继续口服给药 4 天。组织学结果、抗炎和抗氧化应激生物标志物显示，与溃疡组相比，菌株具有保护作用。作为结论，我们介绍了用于药物制剂和健康应用的三种新型益生菌候选物。